产品名称
表皮生长因子 来源于鼠颔下腺, EGF, suitable for cell culture
biological source
mouse submaxillary glands
form
lyophilized powder
potency
0.1-10 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
6 kDa
packaging
pkg of 0.1 and 5X0.1 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤50 EU/vial (LAL test)
color
white to off-white
solubility
water: soluble, clear, colorless (1 vial is reconstituted with 0.5 mL of 0.85% NaCl)
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
mouse ... Egf(13645)
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Disclaimer
该产品应2-8°C保存。复溶后可在2至-8°C下储存一个月；分装冷冻后可在-70至-20℃下储存更久。
Analysis Note
该产品的生物活性是通过测量它激活EGF-反应小鼠BALB/MK细胞的能力确定的。EC50被定义为在基于细胞的生物测定中可引起细胞生长增加50％的生长因子有效浓度。
Application
来自鼠上颌下腺的表皮生长因子(EGF)已用作促细胞分裂剂，触发多种细胞系启动细胞有丝分裂。在组织培养中，EGF也可以降低或消除对血清的需求，还可以和其他培养基添加剂和激素共用。EGF工作浓度通常为0.1-10ng/ml。
Biochem/physiol Actions
EGF是各种表皮和上皮细胞的分裂素，这些细胞包括成纤维细胞，胶质细胞，血管和角膜内皮细胞，牛颗粒细胞，HeLa细胞，SV40-3T3细胞，和哺乳动物上皮细胞，兔软骨细胞。EGF还能够抑制胃酸分泌，在胚胎阶段支持生长和中枢神经系统神经调节。 EGF细胞代谢作用有刺激离子流，葡萄糖转运，糖酵解，DNA，RNA和蛋白质合成。
Other Notes
表皮生长因子（EGF）是在许多哺乳动物物种中的大量组织和体液中都存在的一种小型促有丝分裂多肽。EGF是生长因子家族的一员，其特征在于形成了三个二硫键的6个保守半胱氨酸基序。 人EGF也就是 ß-尿抑胃素，是一种根据它抑制胃酸分泌的性质而分离出来的分子。EGF和人转化生长因子-a结构同源，两者都通过EGF受体发生作用。
Preparation Note
该产品是1mL 5mM乙酸铵溶液，在pH6.5下，冻干粉。它需要加入含有0.1-1.0%BSA或1-10%血清的缓冲液或组织培养基，重悬。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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