E4274
内毒素去除 溶液
Remove lipopolysaccharides (LPS) from plasmid DNA preparations
别名:
endo removal reagent, ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free
产品名称
内毒素去除 溶液, Remove lipopolysaccharides (LPS) from plasmid DNA preparations
Quality Level
Application
内毒素去除溶液已被用来去除面包酵母核糖核酸中的脂多糖。
Biochem/physiol Actions
该试剂依赖于Triton X-114的胶束性质来去除溶液中的内毒素。 当温度高于20°C时，胶束聚集成一个单独的相，隔离两亲性溶质，包括脂多糖。 亲水DNA保留在水相中。
Preparation Note
1体积处理5体积的脱氧核糖核酸溶液（& # 8804；1毫克/毫升）
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Determination of endotoxin through an aptamer-based impedance biosensor.
Su W
Biosensors And Bioelectronics, 32(1), 32-36 (2012)
M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)
商品
FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.
相关内容
Datasheet
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