E4643
肝素结合EGF 样生长因子 人
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
别名:
hHB-EGF, HB-EGF, 肝素结合表皮生长因子样生长因子
登录并加购，请在购物车里查看协议价、货期和发货地。
选择规格
变更视图
关于此项目
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Assay:
≥97% (SDS-PAGE and N-terminal analysis)
Form:
lyophilized powder
Mol wt:
9.5 kDa
产品名称
肝素结合EGF 样生长因子 人, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in baculovirus infected Sf21 cells
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
≤3 ng/mL
quality
endotoxin tested
mol wt
9.5 kDa
packaging
pkg of 50 and 250 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
General description
Heparin‑Binding Epidermal Growth Factor‑Like Growth Factor (HB‑EGF) is a recombinant human member of the epidermal growth factor (EGF) family, expressed in baculovirus‑infected Sf21 insect cells and supplied as a lyophilized powder. HB‑EGF is produced with high purity (≥97%) and is suitable for use in mammalian cell culture and in vitro research applications.
Application
Recombinant human HB‑EGF is commonly used in cell‑based research applications, including: •Mammalian cell culture studies
•Growth and signaling studies in EGF‑responsive cell lines
•Cellular proliferation and migration assays in in vitro model systems
•Growth and signaling studies in EGF‑responsive cell lines
•Cellular proliferation and migration assays in in vitro model systems
Biochem/physiol Actions
与BTC一样，肝素结合表皮生长因子（HB-EGF）是肝素结合EGF家族的成员。天然可溶性HB-EGF是一种19 kDa的糖蛋白，因异质性截短而具有多种形式。可溶性HB-EGF对成纤维细胞、角质形成细胞和平滑肌细胞具有促有丝分裂和趋化作用，但对毛细血管内皮细胞则没有。在单核细胞、巨噬细胞以及血管和主动脉平滑肌细胞（SMC）中可检测到HB-EGF的产生。HB-EGF在动脉粥样硬化的发病机理中可能会起作用。它也可能是Jun诱导的致癌转化的效应因子。HB-EGF可以高亲和力与EGFR结合，并以中等亲和力与ErbB结合。
Physical form
从含有2.5 mg牛血清白蛋白并经0.2 μm滤膜过滤的磷酸盐缓冲盐中冻干。
Analysis Note
生物活性通过它在EGF反应性BALB/3T3细胞中刺激3H-胸苷掺入的能力来测量。
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
flash_point_f
No data available
flash_point_c
No data available