E6282
Anti-eIF2Bβ (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-EIF-2B, Anti-EIF2B-Beta, Anti-EIF2BB, Anti-Eukaryotic translation initiation factor 2B, Beta, Anti-Eukaryotic translation initiation factor 2B, subunit 2
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
4
Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~39 kDa
species reactivity
human, rat, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 3-5 μg using K562 cell lysates, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ, indirect immunofluorescence: suitable, western blot: 2-4 μg/mL using K562 or AT3B1 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EIF2B2(8892)
mouse ... Eif2b2(217715)
rat ... Eif2b2(84005)
General description
Translation initiation factor eIF-2B subunit beta eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.
Application
Anti-eIF2Bβ (N-terminal) antibody produced in rabbit is suitable for the following applications:
- Immunoprecipitation at a concentration of 3-5μg using K562 cell lysates
- Indirect immunofluorescence (at a concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ)
- Western blotting at a concentration of 2-4μg/mL using K562 or AT3B1 cell lysates
Biochem/physiol Actions
Anti-eIF2BNβ-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.
Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease. The eIF2B -α, -β and - δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51.
Translation initiation factor eIF-2B subunit β is a protein encoded by EIF2B2 gene in humans. It is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. It is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Yukiko Hata et al.
International journal of clinical and experimental pathology, 7(6), 3355-3362 (2014-07-18)
We report a rare autopsy case of early infantile-onset vanishing white matter disease, with a submicroscopic deletion of 14q24.3, which included EIF2B2 and a missense mutation of EIF2B2 (V85E) of the remaining allele. The patient was a 4-year-old boy, who
G D Pavitt
Biochemical Society transactions, 33(Pt 6), 1487-1492 (2005-10-26)
eIF2B (eukaryotic initiation factor 2B) is a multisubunit protein that is required for protein synthesis initiation and its regulation in all eukaryotic cells. Mutations in eIF2B have also recently been found to cause a fatal human disease called CACH (childhood
W Yang et al.
Molecular and cellular biology, 16(11), 6603-6616 (1996-11-01)
Eukaryotic translation initiation factor 2B (eIF2B) is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. Phosphorylation of the alpha subunit of eIF2 [creating eIF2(alphaP]) converts eIF2 x GDP from a substrate to an inhibitor of eIF2B. We showed
Andreas Ohlenbusch et al.
Human mutation, 25(4), 411-411 (2005-03-19)
Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities with a wide continuum of clinical signs and severity leading to the concept of eIF2B-related disorders. The clinical
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