shelf life
1 yr at 2‑8 °C
analyte chemical class(es)
proteins (c-Myc)
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
capacity
~0.05 mg/mL binding capacity
storage temp.
2-8°C
Quality Level
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
Application
EZview™红色c-Myc抗体亲和凝胶适于：
- SUMO化实验
- 亲和捕获（免疫沉淀）来自大肠杆菌或哺乳动物细胞的N端或C端c-Myc标记融合蛋白。
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。
Features and Benefits
- 提高能见度 - 红色指示可降低意外吸取沉淀的风险
- 通过减少意外损失，提高目标蛋白质的回收率
- 更高的重现性 - 更稳定的得率
General description
EZ view 红色c-Myc亲和凝胶抗体是一种树脂，由抗c-Myc兔抗与4％琼脂糖珠共价结合而形成。亲和凝胶用于将c-Myc融合蛋白与样品（如细胞裂解物和组织）结合，以便在免疫沉淀测定准备过程中纯化c-Myc标签蛋白。红色染料可增强能见度，从而获得更有效的结果。
琼脂糖珠与大肠杆菌（E. coli）和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。
琼脂糖珠与大肠杆菌（E. coli）和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。
Other Notes
亲和凝胶含有与琼脂糖珠共价结合的亲和纯化c-Myc兔多克隆抗体。 c-Myc标签对应于人c-Myc蛋白的氨基酸残基410-419（EQKLISEEDL）。
Physical form
溶剂为PBS的1:1(v/v)悬浮液，含有50％甘油和0.0015％Kathon CG/IPCII作为抗菌防腐剂
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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