产品名称
环氧基-活化-琼脂糖凝胶 ™ 6B, lyophilized powder
form
lyophilized powder
extent of labeling
19-40 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 6B
matrix active group
epoxy
matrix attachment
through epoxy to hydroxyl
matrix spacer
12 atoms
swelling
1 g swells to 3.0-5.0 mL
particle size
45—165 μm
storage temp.
2-8°C
Quality Level
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Application
环氧活化琼脂糖凝胶™用于亲和层析、蛋白层析和活化/功能化基质。环氧活化琼脂糖凝胶™已用于获得抗原核表达的口蹄疫病毒 (FMDV) 非结构蛋白 (NSP) 3B 的单克隆抗体，以及研究 B 脑膜炎球菌病。
General description
配体与游离环氧基偶联。连接形成稳定的不带电醚。
Other Notes
配体：1,4-双（2:3-环氧丙氧基）丁烷
连接：一个环氧基
间隔基：当配体与游离环氧基偶联时，提供 12 原子间隔基。连接是一种稳定的不带电的醚。
连接：一个环氧基
间隔基：当配体与游离环氧基偶联时，提供 12 原子间隔基。连接是一种稳定的不带电的醚。
Physical form
冻干粉末
Legal Information
Sepharose is a trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Dong Li et al.
Veterinary research communications, 34(5), 445-457 (2010-06-01)
A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then
C A O'Brian et al.
Cancer research, 48(13), 3626-3629 (1988-07-01)
We have previously demonstrated that tamoxifen and related triphenylethylene compounds are potent inhibitors of protein kinase C (PKC). The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose
Kasim Abass Askar et al.
Applied biochemistry and biotechnology, 165(1), 336-346 (2011-04-19)
The purpose of this study was to develop a protocol for the purification of acetylcholinesterase (AChE, acetylcholine acetylhydrolase, E.C.3.1.1.7) enzyme and to extend a purification method for further enzyme characterization. A further aim was to study whether the edrophonium's pharmacologic
Adam Frankel et al.
RNA (New York, N.Y.), 9(7), 780-786 (2003-06-18)
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino
M G Marcu et al.
Journal of the National Cancer Institute, 92(3), 242-248 (2000-02-03)
Heat shock protein 90 (Hsp90) interacts with and stabilizes several oncogenic protein kinases (e.g., p185(erbB2), p60(v-src), and Raf-1) and is required for the stability and dominant-negative function of mutated p53 protein. Two unrelated antibiotics, geldanamycin and radicicol, bind specifically to
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