Merck
CN

E7637

Sigma-Aldrich

溴化乙锭

BioReagent, for molecular biology, powder

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别名:
3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵
经验公式(希尔记法):
C21H20BrN3
CAS号:
分子量:
394.31
Beilstein:
3642536
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.31

等级

for molecular biology

质量水平

产品线

BioReagent

形式

powder

技术

electrophoresis: suitable

mp

260-262 °C (dec.) (lit.)

荧光

λex 360 nm; λem 590 nm in PBS
λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;)
λex 526 nm; λem 605 nm in aqueous buffer

适用性

suitable for gel electrophoresis

SMILES字符串

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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相关类别

应用

溴化乙锭 (EtBr) 是 PAGE 或琼脂糖凝胶电泳最常用的核酸染色剂。EtBr 的荧光在与双链 RNA 结合后可增加 21 倍,在与双链 DNA 结合后增加 25 倍,因此在低染色浓度(10μg/ml)下无需本底脱色。溴化乙锭已用于大量核酸荧光测定。 它已被证明可与单链 DNA(虽然没有那么强)和三链 DNA 结合。由于它能与 DNA 结合,EtBr 是 DNA 聚合酶的抑制剂。

插入双链 DNA 和 RNA 的移码诱变剂。

生化/生理作用

溴化乙啶可插入双链 DNA 和 RNA 中,并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

重悬

对于在电泳后进行凝胶染色,应先用水将储存液样品稀释到 0.5μg/ml,再将凝胶孵育 15-30 分钟。通常无需脱色,但是如果必须降低背景色,则可在水中脱色 15 分钟。然后可在紫外灯箱(254nm 波长)中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液(终浓度 0.5μg/ml)中,并在电泳后立即显影。

相关产品

产品编号
说明
价格

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险声明

危险分类

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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