biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 1-2 μg using HeLa cell lysates., western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ETF1(2107)
mouse ... Etf1(225363)
rat ... Etf1(307503)
General description
Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved.
Application
Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site.
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
相关内容
Instructions
Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division
Petsch K, et al.
Plant Physiology, 139(1), 115-126 (2005)
Identification and characterization of a novel ubiquitous nucleolar protein `NARR?encoded by a gene overlapping the rab34 oncogene
Zougman A, et al.
Nucleic Acids Research, 39(16), 7103-7113 (2011)
L Frolova et al.
Nature, 372(6507), 701-703 (1994-12-15)
The termination of protein synthesis in ribosomes is governed by termination (stop) codons in messenger RNAs and by polypeptide chain release factors (RFs). Although the primary structure of prokaryotic RFs and yeast mitochrondrial RF is established, that of the only
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E8156-200UL | 04061837771330 |