Anti-E2F3 antibody ,Mouse monoclonal

Monoclonal Anti-E2F3 (mouse IgG2a isotype) is derived from the PG30 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human E2F Transcription Factor 3 (E2F3) protein. Transcription factor E2F3 is a DNA-binding protein of ~57 kDa encoded by the E2F3 gene in humans. It belongs to E2F transcription factor family of proteins that bind to the sequence TTTCGCGC.

recombinant human E2F3.

Monoclonal Anti-E2F3 antibody produced in mouse has been used for the following applications:

• immunoprecipitation using denatured E2F3
• western blotting at a concentration of 4-8μg/mL using transfected 293T cells expressing E2F3
• electrophoretic mobility shift assay (EMSA).

Transcription factor E2F3 is involved in the regulation of cell cycle. E2F3 helps in the regulation of the expression of a number of genes that are important in cell proliferation. It is a oncogene with strong proliferative potential and is involved in many processes. E2F3 plays an important role in the development of several types of cancer. Overexpression of E2F3 may be associated with hepatocellular carcinoma (HCC). It plays an important role in controlling cell cycle progression and proliferation. E2F3 is frequently expressed in pediatric Wilms′ tumors and may be associated with the same.

Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

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