E9030
Endoglycoceramidase II from Rhodococcus sp.
aqueous solution
别名:
EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase
recombinant
expressed in E. coli
conjugate
(Lipid-linked)
form
solution
mol wt
58.9 kDa
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.
Physical form
Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.
Other Notes
One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Hiroaki Korekane et al.
Analytical biochemistry, 364(1), 37-50 (2007-03-14)
The structures of acidic glycosphingolipids in colon adenocarcinoma have been analyzed extensively using a number of conventional methods, such as thin-layer chromatography and methylation analysis, and a variety of acidic glycosphingolipids present in the tissues have been reported. However, because
Ryuichi Higuchi et al.
Chemical & pharmaceutical bulletin, 54(3), 287-291 (2006-03-02)
A ganglioside molecular species GP-3 (1) has been obtained from the water-soluble lipid fraction of the chloroform/methanol extract of the starfish Asterina pectinifera. The structure of the ganglioside has been determined on the basis of chemical and spectroscopic evidence. Compound
A E Manzi
Current protocols in molecular biology, Chapter 17, Unit17-Unit17 (2008-02-12)
Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used
Nahid Azzouz et al.
International journal for parasitology, 32(6), 677-684 (2002-06-14)
Glycolipids are important components of cellular membranes involved in various biological functions. In this report, we describe the identification of the de novo synthesis of glycosphingolipids by Toxoplasma gondii tachyzoites. Parasite-specific glycolipids were identified by metabolic labelling of parasites with
Matthew E C Caines et al.
The Journal of biological chemistry, 282(19), 14300-14308 (2007-03-03)
endo-Glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of the family, preferentially hydrolyzing glycosidic linkages between the oligosaccharide and ceramide moieties of gangliosides. Here we report the first x-ray crystal structures
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