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Merck
CN

E9640

Endothelial cell growth factor

100 ×, sterile-filtered, suitable for cell culture

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UNSPSC Code:
12352202
Biological source:
bovine pituitary glands
Impurities:
endotoxin, tested
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biological source

bovine pituitary glands

sterility

sterile-filtered

packaging

pkg of 1 L, pkg of 10 mL, pkg of 5 mL

storage condition

avoid repeated freeze/thaw cycles

concentration

100 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−70°C

Quality Level

Application

Endothelial Cell Growth Factor (ECGF) has been optimized for the culture of Human Umbilical Vein Endothelial Cells (HUVEC). ECGF is optimized for the mitogenic proliferation of HUVEC cells when supplemented to CS-C Medium. ECGF should be supplemented to CS-C medium at a concentration 1 ml per 100 ml of culture medium. ECGF is prepared in DPBS (without calcium and magnesium) supplemented with porcine heparin and HBGF-1. For best results it is recommended that this product be used in conjugation with CS-C Medium for Endothelial Cell Lines (Product Nos. C 1431 or C 1556), Endothelial Attachment Factor (Product No. E 9765), Trypsin/EDTA Solution for Endothelial Cell Cultures (Product No. T 4299) and Trypsin Inhibitor Solution (Product No. T 6414).

Preparation Note

5 mg/ml each porcine heparin and HBGF-1 in DPBS without calcium and magnesium

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

新产品
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分析证书(COA)

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