biological source
bovine pituitary glands
Quality Segment
sterility
sterile-filtered
packaging
pkg of 1 L, pkg of 10 mL, pkg of 5 mL
storage condition
avoid repeated freeze/thaw cycles
concentration
100 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−70°C
Application
Endothelial Cell Growth Factor (ECGF) has been optimized for the culture of Human Umbilical Vein Endothelial Cells (HUVEC). ECGF is optimized for the mitogenic proliferation of HUVEC cells when supplemented to CS-C Medium. ECGF should be supplemented to CS-C medium at a concentration 1 ml per 100 ml of culture medium. ECGF is prepared in DPBS (without calcium and magnesium) supplemented with porcine heparin and HBGF-1. For best results it is recommended that this product be used in conjugation with CS-C Medium for Endothelial Cell Lines (Product Nos. C 1431 or C 1556), Endothelial Attachment Factor (Product No. E 9765), Trypsin/EDTA Solution for Endothelial Cell Cultures (Product No. T 4299) and Trypsin Inhibitor Solution (Product No. T 6414).
Preparation Note
5 mg/ml each porcine heparin and HBGF-1 in DPBS without calcium and magnesium
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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