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E9644

Epidermal Growth Factor, human

Name: Epidermal Growth Factor, human
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

表皮生长因子人, EGF

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关于此项目

化学文摘社编号:

62253-63-8

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00132565

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

~6 kDa

Impurities:

≤1 EU/μg endotoxin (EGF)

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产品名称

重组人表皮细胞生长因子, EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

300

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.08-0.8 ng/mL ED₅₀/EC₅₀

mol wt

~6 kDa

packaging

pkg of 5X0.2 mg, pkg of 0.2 mg, pkg of 0.5 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin (EGF)

color

white

solubility

water: soluble, clear, colorless

UniProt accession no.

P01133

storage temp.

−20°C

SMILES string

S(CC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]%12N(CCC%12)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N

InChI

1S/C270H401N73O83S7/c1-24-134(19)217(262(420)293-112-201(355)298-161(69-74-205(359)360)229(387)301-160(45-35-82-286-269(279)280)228(386)333-192(119-428)255(413)307-162(68-73-198(274)352)230(388)316-176(94-141-54-64-150(350)65-55-141)241(399)304-159(44-34-

InChI key

GVUGOAYIVIDWIO-UFWWTJHBSA-N

Gene Information

human ... EGF(1950)

Application

该产品用作促分裂原，在各种细胞系中引发细胞开始有丝分裂。在组织培养中，EGF可用于减少或消除对血清的需求，并可与其他培养基添加剂和激素一起使用。

Biochem/physiol Actions

表皮生长因子（EGF）是在许多哺乳动物物种中的大量组织和体液中都存在的一种小型促有丝分裂多肽。EGF是生长因子家族的一员，其特征在于形成了三个二硫键的6个保守半胱氨酸基序。EGF对多种表皮和上皮细胞具有促有丝分裂作用，包括成纤维细胞、神经胶质细胞、血管和角膜内皮细胞、牛颗粒细胞、HeLa细胞、SV40-3T3细胞和乳腺上皮细胞。
受EGF影响的细胞功能：有丝分裂、离子通量、葡萄糖转运、糖酵解、核酸及蛋白质合成、存活、生长、增殖和分化。
EGF的生物学影响：抑制胃酸分泌、胎儿生长发育和中枢神经系统的神经调节。
受EGF影响的通路：EGFR信号通路，MAPK级联反应通路，PIP，Ca+2信号通路

Preparation Note

该产品是由经0.2 μm-过滤的pH 7.4磷酸盐缓冲液冻干而得。它应该通过使用10mM 乙酸将小瓶中的内容物加入至1mg / mL来进行重悬。如需稀释或更低的浓度，但不低于10 μg/mL时，需要加入0.1% BSA或HSA。如用于增殖检测，则可通过不含白蛋白的培养基对其进一步稀释。

Analysis Note

该产品的生物学活性是通过在BALB/MK细胞中进行的增殖试验而得以测量的。EC₅₀被定义为在基于细胞的生物测定中可引起细胞生长增加50％的生长因子有效浓度。

Other Notes

人EGF（hEGF）是与β-尿抑胃素相同的分子，而这是一种基于其能够抑制胃酸分泌的能力而分离的分子。EGF在结构上与人转化生长因子-α同源，而二者都可以通过EGF受体发挥其作用。 E9644是天然成熟EGF的N-末端甲硫氨酸形式。

Disclaimer

该产品应当储存在-20°C并将保持活性长达两年。在重悬后，它可在2至-8°C下储存一个月，或在分装冷冻后在-70至-20℃下储存更长时间。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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全球贸易项目编号

货号	GTIN
E9644-.2MG	04061838447111
E9644-5X.2MG	04061835568437
E9644-.5MG	04061838447128

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