产品名称
Anti-Endothelial Cells antibody, Mouse monoclonal, clone P1H12, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P1H12, monoclonal
form
buffered aqueous solution
mol wt
antigen 130 kDa
species reactivity
human
technique(s)
flow cytometry: suitable using 2% paraformaldehyde 9:1 methanol
immunocytochemistry: suitable using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells
immunohistochemistry: suitable using using acetone-fixed sections of human tongue
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
Anti-Endothelial Cells antibody, Mouse monoclonal has been used in:
- immunocytochemistry
- immunohistochemistry
- flow cytometry
- immunoprecipitation
- enzyme-linked immunosorbent assay (ELISA)
- western blotting
Biochem/physiol Actions
CD146 (cluster of differentiation 146) expression can stimulate tumor development in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells during implantation and placentation, thus limiting trophoblastic invasion in the myometrium.
Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation.
Soluble CD146 (cluster of differentiation 146) is associated with the activation of angiogenesis. Circulating endothelial cells (CECs) may help in predicting clinical outcome in cancer patients. Tumor endothelial markers are expressed in a wide range of tumor types and in normal vessels associated with wound healing and corpus luteum formation. It may have significant implications for the development of anti-angiogenic therapies as well.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Endothelial cells (CD146) (mouse IgG1 isotype) is derived from the P1H12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with human umbilical cord cells (HUVEC). CD146 (cluster of differentiation 146) is also known as the melanoma cell adhesion molecule (MCAM), cell surface glycoprotein MUC18, A32, Mel-CAM and S-Endo-1. It is encoded by the MCAM gene. It belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. It is located on human chromosome11q23.
Immunogen
human umbilical cord cells (HUVEC).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Expression of CD markers in JAK2V617F positive myeloproliferative neoplasms: Prognostic significance
Shahrabi S, et al.
Oncology reviews, 12(2) (2018)
The role of CD146 (Mel-CAM) in biology and pathology
Shih IM
The Journal of Pathology, 189(1), 4-11 (1999)
Xianyi Yu et al.
Pediatric research, 55(4), 688-694 (2004-02-07)
Nitric oxide (NO) serves many vasoprotective roles, but the massive release of NO causes arterial wall degeneration. We investigated whether enhanced nitric oxide synthase (iNOS) expression in peripheral blood leukocytes and circulating endothelial cells mirrors the progression of coronary arterial
Targeting CD146 with a 64Cu-labeled antibody enables in vivo immunoPET imaging of high-grade gliomas
Yang Y, et al.
Proceedings of the National Academy of Sciences of the USA, 112(47), E6525-E6534 (2015)
M Ilie et al.
British journal of cancer, 110(5), 1236-1243 (2014-01-30)
Previous studies indicate that endothelial injury, as demonstrated by the presence of circulating endothelial cells (CECs), may predict clinical outcome in cancer patients. In addition, soluble CD146 (sCD146) may reflect activation of angiogenesis. However, no study has investigated their combined
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