E9762
Endoglycosidase F1
Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli
别名:
Endo F1, 内切-β-N-乙酰氨基葡萄糖苷酶F1, 内切糖苷酶F1 来源于脑膜炎脓毒性黄杆菌, 内切糖苷酶F1 来源于脑膜脓毒性伊丽莎白菌, 内切糖苷酶F1 来源于脑膜脓毒性金黄杆菌
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关于此项目
Specific activity:
≥16 U/mg
Recombinant:
expressed in E. coli
产品名称
内切糖苷酶F1 from Elizabethkingia miricola, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution
recombinant
expressed in E. coli
Quality Level
conjugate
(N-linked)
form
buffered aqueous solution
specific activity
≥16 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
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General description
来源于米尔伊丽莎白菌的糖苷内切酶F1是一种聚糖特异性酶。
Application
来源于米尔伊丽莎白菌的糖苷内切酶F1已被用于解除人血色素沉着蛋白 (HFE) 与阳离子非依赖性甘露糖-6-磷酸受体 (CI-MPR) 的结合并用于岩藻糖基化 N-糖肽的糖基化。
切割天冬酰胺连接或游离的低聚甘露糖、以及杂化但不是复合的低聚糖。
Biochem/physiol Actions
来源于米尔伊丽莎白菌的糖苷内切酶F1在蛋白质聚糖结构中壳二糖核心的两个N-乙酰葡糖胺残基的裂解中起到重要作用。它介导高甘露糖和杂交寡聚糖的裂解。
Packaging
提供5×反应缓冲液，250mM NaH2PO4，pH 5.5。
Physical form
在20mM Tris-HCl（pH 7.5）中无菌填充的溶液
Other Notes
在37℃，pH5.5条件下，一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Receptor activity-modifying protein 1 determines the species selectivity of non-peptide CGRP receptor antagonists
Mallee JJ, et al.
The Journal of Biological Chemistry, 277, 14294-14298 (2002)
EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and alpha-acid glycoprotein
Jonathan S, et al.
BioChemistry: An Indian Journal, 455, 107-118 (2013)
In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor
Schimanski LM, et al.
Blood Cells, Molecules and Diseases, 43(2), 180-193 (2009)
Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM
Ma J, et al.
Journal of proteomics, 189, 67-74 (2018)
M V Padkina et al.
Prikladnaia biokhimiia i mikrobiologiia, 46(4), 448-455 (2010-09-29)
The HuIFNA16, HuIFNB, and BoIFNG genes encoding human [alpha]16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed. There was no
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
相关内容
Instructions
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E9762-1UN | 04061833609200 |
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