E9762
Endoglycosidase F1
Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli
别名:
Endo F1, 内切-β-N-乙酰氨基葡萄糖苷酶F1, 内切糖苷酶F1 来源于脑膜炎脓毒性黄杆菌, 内切糖苷酶F1 来源于脑膜脓毒性伊丽莎白菌, 内切糖苷酶F1 来源于脑膜脓毒性金黄杆菌
产品名称
内切糖苷酶F1 from Elizabethkingia miricola, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution
recombinant
expressed in E. coli
conjugate
(N-linked)
form
buffered aqueous solution
specific activity
≥16 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
来源于米尔伊丽莎白菌的糖苷内切酶F1是一种聚糖特异性酶。
Application
切割天冬酰胺连接或游离的低聚甘露糖、以及杂化但不是复合的低聚糖。
来源于米尔伊丽莎白菌的糖苷内切酶F1已被用于解除人血色素沉着蛋白 (HFE) 与阳离子非依赖性甘露糖-6-磷酸受体 (CI-MPR) 的结合并用于岩藻糖基化 N-糖肽的糖基化。
Biochem/physiol Actions
来源于米尔伊丽莎白菌的糖苷内切酶F1在蛋白质聚糖结构中壳二糖核心的两个N-乙酰葡糖胺残基的裂解中起到重要作用。它介导高甘露糖和杂交寡聚糖的裂解。
Packaging
提供5×反应缓冲液，250mM NaH2PO4，pH 5.5。
Physical form
在20mM Tris-HCl（pH 7.5）中无菌填充的溶液
Other Notes
在37℃，pH5.5条件下，一个单位可在每分钟内从1 μmole变性核糖核酸酶B中释放N-连接的寡糖。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Receptor activity-modifying protein 1 determines the species selectivity of non-peptide CGRP receptor antagonists
Mallee JJ, et al.
The Journal of Biological Chemistry, 277, 14294-14298 (2002)
EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and alpha-acid glycoprotein
Jonathan S, et al.
BioChemistry: An Indian Journal, 455, 107-118 (2013)
In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor
Schimanski LM, et al.
Blood Cells, Molecules and Diseases, 43(2), 180-193 (2009)
Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM
Ma J, et al.
Journal of proteomics, 189, 67-74 (2018)
Yusuke Tomabechi et al.
Carbohydrate research, 345(17), 2458-2463 (2010-10-12)
To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
相关内容
Instructions
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