E9906
Anti-EDEM2 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-ER degradation enhancer, mannosidase alpha-like 2
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关于此项目
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
2
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
human, rat (predicted), mouse
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells, western blot: 0.5-1 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EDEM2(55741)
mouse ... Edem2(108687)
rat ... Edem2(296304)
General description
ER degradation-enhancing α-mannosidase-like 2 is an enzyme encoded by the EDEM2 gene in humans. EDEM2 is localized to the endoplasmic reticulum (ER) mainly as a soluble glycoprotein. It is an enzyme encoded by the EDEM2 gene in humans. It is one of the ER-stress-induced members of the glycosyl hydrolase 47 family.
Immunogen
synthetic peptide corresponding to amino acids 22-38 of human EDEM2, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and 2 amino acids in rat EDEM2.
Application
Anti-EDEM2 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence at a concentration of 5-10μg/mL using mouse 3T3 cells and western blotting at a concentration of 0.5-1μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2.
Biochem/physiol Actions
Overexpression of ER degradation-enhancing alphamannosidase-like protein 2 (EDEM2) accelerates ER associated degradation (ERAD) by promoting the release of terminally misfolded glycoproteins from the calnexin cycle, without affecting the rate of degradation of nonglycosylated polypeptides or the maturation of model secretory proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle
Molinari M, et al.
Science, 299(5611), 1397-1400 (2003)
Steven W Mast et al.
Glycobiology, 15(4), 421-436 (2004-11-13)
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the
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