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Merck
CN

ECM001

Human Fibronectin

recombinant, expressed in HEK 293 cells, powder, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞, CIG, FN, 冷不溶性球蛋白

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00131062

主要文件

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产品名称

人纤维连接蛋白, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

biological source

human

recombinant

expressed in HEK 293 cells

sterility

sterile

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)

packaging

pkg of 200 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin, tested

solubility

water: soluble

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

shipped in

ambient

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FN1(2335)

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相关类别

Analysis Note

通过测定固定化纤维连接蛋白支持 CHO 细胞黏附的能力，检测重组人纤连蛋白在培养中的生物活性。

Application

上皮细胞，间充质细胞，神经元细胞，成纤维细胞，神经嵴细胞，内皮细胞。
推荐用作细胞培养基质，1-5μg/cm 2 或 0.5-50μg/ml。最佳浓度取决于细胞类型以及应用或研究目的。
人纤连蛋白用于包被细胞培养板，培养内皮祖细胞。它还作为Dulbecco′s改良Eagle′培养基（DMEM）成分，用于胶质母细胞瘤细胞的 体外 人工基膜侵袭试验。

Biochem/physiol Actions

纤连蛋白（FN）对胚胎发育至关重要，介导细胞的粘附和迁移功能。介导鼻咽癌（NPC）细胞凋亡。血浆FN调节血栓形成。纤连蛋白可作为整联蛋白受体家族 的配体，包含胶原蛋白、糖蛋白和糖胺聚糖结合位点。 FNI基因突变造成纤连蛋白的可溶形式和不可溶形式失衡，导致遗传性肾脏疾病-肾小球病中的肾小球沉积。FN1基因的错义突变与骨骼发育不良疾病-脊柱干骺端发育不良有关。

Features and Benefits

  • 人源纤连蛋白
  • 通过HEK 293细胞中的重组表达生产
  • 内毒素水平低
  • 无细菌污染

General description

纤连蛋白（FN）是糖蛋白，5％-9％的糖类以 N-和 O-连接寡糖的形式存在。 属于配体糖蛋白家族。FN基因位于人类染色体2q35。FN以两种主要的同工型存在，即，基于血浆的可溶形式和基于细胞外基质（ECM）的不溶形式。纤连蛋白结构包含FNI、FNII和FNIII重复序列的重复单元。它还具有血纤蛋白结合位点，以二聚体形式存在。FN1基因的可变剪接导致产生许多同工型。该纤连蛋白是用全人类生产系统在人细胞中生产，不含血清。该人类细胞表达系统可实现与人类蛋白相仿的糖基化和折叠，通常可为培养物中的蛋白提供更好的稳定性支持。

Physical form

以粉末形式提供，由盖缓冲盐水冻干。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000233596
0000233596
0000154101
0000106218

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Anna Lemańska-Perek et al.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 28(11), 1561-1567 (2019-10-10)
Fibronectin (FN) is a widely distributed glycoprotein which is present in different bodily fluids, on the surface of cells and in the extracellular matrix (ECM). It plays roles in various processes, including cell adhesion, migration, growth, proliferation, and tissue repair.
Jeannette Huaman et al.
Cells, 9(7) (2020-07-08)
Fibronectin (FN1) is an extracellular matrix protein gaining increasing attention for its multifaceted roles in cancer progression. Using our recently established circulating tumor cell (CTC) lines, we had demonstrated increased FN1 expression and enhanced migration in CTC lines, in comparison
Hsiang-Chi Tseng et al.
Nature communications, 11(1), 4810-4810 (2020-09-25)
Chimeric antigen receptor (CAR) therapy is a promising immunotherapeutic strategy for treating multiple refractory blood cancers, but further advances are required for solid tumor CAR therapy. One challenge is identifying a safe and effective tumor antigen. Here, we devise a
Jinting Wang et al.
American journal of translational research, 9(10), 4502-4511 (2017-11-10)
Fibronectin 1 (FN1) is a member of the glycoprotein family located on chromosome 2q35. It has been reported that FN1 is upregulated in many tumors, and its expression is negatively related to the prognosis and survival of cancer patients. Through
The association between hyperhomocysteinemia and cerebral small vessel disease
Yang Y, et al.
International Journal of Clinical and Experimental Medicine, 12, 13877-13883 (2019)
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细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

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实验方案

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Instructions

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