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Sigma-Aldrich

酶蛋白去糖基化试剂盒

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别名:
Glycosylation Release Kit
NACRES:
NA.32

偶联物

(N-linked)
 (O-linked)

质量水平

200

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

酶蛋白去糖基化试剂盒包含从糖蛋白中完整去除N连接和单一O连接碳水化合物所需的所有酶和试剂。该试剂盒用于裂解Core 2 O连接碳水化合物复合物，包括含有聚乳糖胺的碳水化合物。

应用

酶促蛋白去糖基化试剂盒用于：
  • 蛋白质去糖基化
  • 组织因子途径抑制因子(TFPI)去糖基化α以及β
  • 天然exo-β-(1,3)-葡聚糖酶EXG1的去糖基化

酶解蛋白去糖基化试剂盒已用于各种酶的去糖基化。

特点和优势

酶蛋白去糖基化试剂盒具有以下特点：
  • 适用于两mg以上规模的糖蛋白去糖基化
  • 中性pH值下单步反应
  • 天然&变性过程
  • 蛋白质无降解
  • 去除含聚唾液酸的O连接糖
  • 随附对照糖蛋白

仅试剂盒组分

产品编号
说明
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
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相关产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Misun Park et al.
Scientific reports, 7(1), 7890-7890 (2017-08-13)
β1-adrenergic receptors (β1ARs) mediate catecholamine actions in cardiomyocytes by coupling to both Gs/cAMP-dependent and Gs-independent/growth-regulatory pathways. Structural studies of the β1AR define ligand-binding sites in the transmembrane helices and effector docking sites at the intracellular surface of the β1AR, but
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International journal of peptides, 2012, 621329-621329 (2012-04-27)
Histoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample
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Journal of microbiological methods, 175, 105981-105981 (2020-06-15)
The diagnosis of histoplasmosis depends on various approaches: direct clinical examination, fungus isolation from cultures of clinical samples, histopathological evaluation, and serological testing. In serodiagnostic assays, the Histoplasma capsulatum H and M antigenic glycoproteins have been extensively used. However, both
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