Merck
CN

EMS0004

Sigma-Aldrich

SOLu-胰蛋白酶

recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

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别名:
用于蛋白酶解的胰蛋白酶, 质谱分析蛋白酶解

重组

expressed in Pichia pastoris

质量水平

等级

Proteomics Grade

描述

Activity:≥ 10,000 unit/mg protein

形式

ready-to-use solution

适用性

suitable for mass spectrometry

UniProt登记号

运输

wet ice

储存温度

2-8°C

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此商品
EMS0005EMS0007T6567
SOLu-Trypsin recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

Sigma-Aldrich

EMS0004

SOLu-胰蛋白酶

Sigma-Aldrich

Sigma-Aldrich

EMS0005

SOLu-Trypsin Dimethylated

grade

Proteomics Grade

grade

Proteomics Grade

grade

Proteomics Grade

grade

-

form

ready-to-use solution

form

ready-to-use solution

form

lyophilized powder

form

-

suitability

suitable for mass spectrometry

suitability

suitable for mass spectrometry

suitability

suitable for mass spectrometry

suitability

-

UniProt accession no.

P00761

UniProt accession no.

P00761

UniProt accession no.

P00761

UniProt accession no.

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

一般描述

胰蛋白酶是一种胰腺丝氨酸内切蛋白酶。SOLu-胰蛋白酶由重组猪胰蛋白酶序列制备而成。 它不具有糜蛋白酶活性(chymotryptic activity)。

应用

胰蛋白酶:
  • 已用于消化凝胶碎片,进行活性去泛素化酶( deubiquitnase,DUB)的质谱鉴定
  • 已用于胰蛋白酶灵敏度测定
  • 可用于消化葡萄酒蛋白质

生化/生理作用

由于胰蛋白酶具有高度特异性裂解能力,胰蛋白酶肽数量有限,因此胰蛋白酶通常用于蛋白质组学研究中的肽定位和蛋白质序列研究。它可特异性地水解精氨酸和赖氨酸残基羧基侧的肽键
  • SOLu-胰蛋白酶(EMS0004)是我们专有的高级蛋白质组学级酶,其在质谱研究中可保持稳定。
  • SOLu-胰蛋白酶经过专门设计,冷藏时其在溶液中稳定,无需制备即可立即使用。
  • 其他形式的胰蛋白酶需要解冻或重溶,如果不立即使用,必须将其丢弃。
  • SOLu-胰蛋白酶可以保留剩余的产品以备将来使用,从而消除了不必要的浪费和成本。
  • 它采用高纯度重组胰蛋白酶配制而成,不含胰凝乳蛋白酶活性,可确保高保真度消化。

特点和优势

  • 即用型-无需重新配制或解冻等准备工作
  • 完美适用于已建立的工作流程-无需修改实验方案
  • 消除浪费-使用后可在冰箱中保持稳定,因此无需丢弃剩余的产品
  • 重组猪序列-无胰凝乳活性
  • 在室温下可在自动进样器或液体处理机器人中短期使用时保持稳定

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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胰蛋白酶,测序级

Mass spectrometry of peptides and proteins using digestion by a grape cysteine protease at pH 3
Perutka Z and Sebela M
Journal of Mass Spectrometry : Jms (2019)
Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications
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Trypsin is by far the most commonly used protease in proteomics. Even though the amount of protease used in each experiment is very small, digestion of large amounts of protein prior to enrichment can be rather costly. The price of
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Cysteine protease from grapevine (Vitis vinifera) belongs to those resistant proteins, which survive the process of vinification and can therefore be detected as wine components. Its amino acid sequence shows a homology to other members of the papain family, but

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