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Merck
CN

F0291

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

成纤维细胞生长因子 7-碱性 人, FGF2, bFGF

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关于此项目

化学文摘社编号:
106096-93-9
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00081382

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产品名称

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤-0.6 ng/mL

mol wt

16.0 kDa

packaging

pkg of 4X25 μg
pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

color

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

P09038

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FGF2 (2247)

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Analysis Note

FGF-Basic 的生物活性是在荧光测定中使用氧化还原敏感型染料刃天青进行测量。

Application

碱性成纤维细胞生长因子（FGF-Basic）是维持培养物中人胚胎干细胞和各种其它干细胞系，如间充质干 （基质） 细胞 （MSC） 所必需的。

General description

碱性成纤维细胞生长因子（FGF-Basic） 存在于基底膜和内皮下细胞外基质中。FGF-Basic 在胚胎发育早期被诱导，它是胚胎干细胞（ESC） 和谱系定义的干细胞的多能性和自我更新所必需的（但不充分）； 其中它起到支持增殖和抑制分化的作用。hESC 的自我更新取决于激活素/Nodal/Smad2,3 分支的激活和 TGFβ 家族细胞信号传导程序的 BMP/GDF/Smad5 分支的抑制。FGF-Basic 是多能性节点支持的感受态因子。FGF-Basic 是维持干细胞特性所必需且充分的。它诱导人 ES 细胞中 Nodal 和 FGF-Basic 的表达。

Physical form

从 20 mM Tris、1 M NaCl（pH 7.0，每1 μg BFGF含 50μg 牛血清白蛋白 (BSA)/1μg 脂联素）的 0.2 μm 过滤溶液冻干。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
胰岛素激素类产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000451315
0000451315
0000195944
0000372843
0000355296

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