F0628
铁氧化还原蛋白-NADP + 还原酶 来源于菠菜
lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
别名:
铁氧还蛋白-NADP+ 氧化还原酶
表单
lyophilized powder
质量水平
比活
≥15 units/mg solid
异质活性
NADH diaphorase activity ≤10 units/mg solid
储存温度
−20°C
一般描述
铁氧还蛋白-NADP+ 还原酶是可溶于铁氧还蛋白的配偶子，与PSI的摩尔比为0.9和3。
应用
Ferredoxin-NADP + 还原酶用于 体外 蓝藻类囊体膜中脂肪酸的铁氧化还原蛋白依赖性去饱和作用。也用于调节甘油醛-3-磷酸脱氢酶。
从 Spinacia oleracea（菠菜）中提取的铁氧还蛋白-NADP+ 还原酶用于试验潜在蛋白配偶子对3-磷酸甘油醛脱氢酶（GAPDH）−CP12制剂活性的影响。它也用于标准测定条件，测定藻蓝素：铁氧还蛋白氧化还原酶（PcyA）酶。
生化/生理作用
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族，该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族，该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
其他说明
在菠菜铁氧还蛋白和 NADP 存在的情况下，在 25℃、ph7.5 的条件下，一个单位将每分钟还原 1.0 毫摩尔的细胞色素 C。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Ana Sánchez-Azqueta et al.
Biochimica et biophysica acta, 1817(7), 1063-1071 (2012-05-01)
The role of the highly conserved C266 and L268 of pea ferredoxin-NADP(+) reductase (FNR) in formation of the catalytically competent complex of the enzyme with NADP(H) was investigated. Previous studies suggest that the volume of these side-chains, situated facing the
Sara Baroni et al.
Biochemistry, 51(18), 3819-3826 (2012-04-24)
Plasmodium falciparum ferredoxin-NADP(+) reductase (FNR) is a FAD-containing enzyme that, in addition to be a promising target of novel antimalarial drugs, represents an excellent model of plant-type FNRs. The cofactor specificity of FNRs depends on differences in both k(cat) and
CRYSTALLIZATION OF FERREDOXIN-TPN REDUCTASE AND ITS ROLE IN THE PHOTOSYNTHETIC APPARATUS OF CHLOROPLASTS.
M SHIN et al.
Biochemische Zeitschrift, 338, 84-96 (1963-01-01)
Shin, M.
Methods in Enzymology, 23, 441-441 (1971)
Manuel Twachtmann et al.
The Plant cell, 24(7), 2979-2991 (2012-07-19)
To adapt to different light intensities, photosynthetic organisms manipulate the flow of electrons through several alternative pathways at the thylakoid membrane. The enzyme ferredoxin:NADP(+) reductase (FNR) has the potential to regulate this electron partitioning because it is integral to most
实验方案
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持