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F0895

Human Fibronectin

Name: Human Fibronectin
Brand: SIGMA

from human plasma, liquid, 1 mg/mL, suitable for cell culture

别名:

纤连蛋白溶液来源于人类成纤维细胞

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关于此项目

UNSPSC Code:

12352202

eCl@ss:

42020141

NACRES:

NA.75

MDL number:

MFCD00131062

Biological source:

human plasma

Form:

liquid

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.1% (Solution)

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属性

产品名称

人血浆纤维连接蛋白, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

biological source

human plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

liquid

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

concentration

0.1% (Solution)

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm²

solubility

saline: 0.05 M (Tris-buffered)

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

说明

General description

纤连蛋白1是由FN1基因编码的细胞外基质糖蛋白。它在血浆和细胞表面表达。FN1 基因位于人染色体2q35。
FN 是一种二聚体蛋白，通过C末端的两个二硫键连接起来。在纤连蛋白结构内部，结构域在接近肽链的氨基和羧基末端的位置紧密排布。此外，研究人员观察到，尽管来源于单个基因，在人体中鉴定出了多达20个不同的基因变异体。

Application

纤连蛋白被应用于测定人血浆H₂O₂ 的消耗速率。它也被用于包被细胞培养用Sylgard^® 模型。它也被用于细胞黏附和将蛋白质直接印在聚合膜上。

Biochem/physiol Actions

纤连蛋白的功能涵盖各种生物过程，包括细胞粘附、生长、迁移、伤口愈合、凝血和转移。纤连蛋白在细胞附着和扩散、细胞骨架控制、细胞形态和分化等方面发挥着重要作用。FN1还参与细胞外基质的形成、止血和血栓形成。FN1基因突变可导致肾小球病变。

Features and Benefits

粘着糖蛋白
来源于人血浆
建议表面覆盖率为 1-5 μg/cm2

Preparation Note

本品为0.1%纤连蛋白溶液。该蛋白的均质性由免疫电泳检测，通过内毒素测试，其源材料HIV、乙肝、丙肝检测均为阴性。

在涂层培养表面，纤连蛋白应在无菌平衡的无菌盐溶液中稀释，并采用最小的涂层体积。表面应在室温下风干45分钟，并可在2-8℃下保存2-4周

Analysis Note

蛋白质均一性由免疫电泳评估。

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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实验方案

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

商品

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.

Altomare L, et al.

The International Journal of Artificial Organs, 33(8), 535-543 (2010)

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Birkenkamp-Demtroder K, et al.

British Journal of Cancer, 105(4), 552-552 (2011)

The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.

Farcas M A, et al.

BioMedical Engineering OnLine, 8(1), 30-30 (2009)

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全球贸易项目编号

货号	GTIN
F0895-1MG	04061833611470
F0895-2MG	04061833611487
F0895-5MG	04061833611494