F1757
Fumarase from porcine heart
ammonium sulfate suspension, ≥300 units/mg protein (biuret)
别名:
L-Malate hydrolyase, Fumarate hydratase
表单
ammonium sulfate suspension
质量水平
比活
≥300 units/mg protein (biuret)
异质活性
malic dehydrogenase ≤0.1%
储存温度
2-8°C
一般描述
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
应用
Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.
生化/生理作用
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
外形
Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
制备说明
Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.
其他说明
One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Fumarase Deficiency: A Safe and Potentially Disease Modifying Effect of High Fat/Low Carbohydrate Diet
Ryder B, et al.
JIMD Reports, 40(3), 77?83-77?83 (2018)
Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid
Giorno L, et al.
Biotechnology and Bioengineering, 72(1), 77-84 (2001)
Fumarase: a paradigm of dual targeting and dual localized functions
Yogev O, et al.
FEBS Journal, 278(22), 4230-4242 (2011)
Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage response
Yogev O, et al.
PLoS Biology, 8(3), e1000328-e1000328 (2010)
Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.
Miettinen M, et al.
American Journal of Surgical Pathology, 40(12), 1661-1669 (2016)
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
Enzymatic Assay of Fumarase
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