Fumarase from porcine heart

Name: Fumarase from porcine heart
Brand: SIGMA

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

别名:

L-Malate hydrolyase, Fumarate hydratase

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关于此项目

化学文摘社编号:

9032-88-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-887-7

MDL number:

MFCD00131111

EC 号:

4.2.1.2 (BRENDA, IUBMB)

Specific activity:

≥300 units/mg protein (biuret)

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属性

form

ammonium sulfate suspension

Quality Level

200

specific activity

≥300 units/mg protein (biuret)

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

说明

General description

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Application

Fumarase from porcine heart has been used:

as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
for immobilized enzyme based stability assay
in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Biochem/physiol Actions

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.