F2055
Monoclonal Anti-hFBXW7 小鼠抗
clone FB407, purified from hybridoma cell culture
别名:
Anti-ARCHIPELAGO (Drosophila), Anti-Cdc4 (S. serevisia), Anti-F-Box and WD40 Domain Protein 7, Anti-FBW7, Anti-SEL-10 (C. elegance)
产品名称
Monoclonal Anti-hFBXW7 小鼠抗, clone FB407, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
FB407, monoclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
rat, human, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FBXW7(55294)
mouse ... Fbxw7(50754)
相关类别
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Application
Anti-hFBXW7 antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.
The antibody recognizes human, mouse, and rat hFBXW7.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.
Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.
Immunogen
synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
The Fbxw7/hCdc4 tumor suppressor in human cancer
Tan YM, et al.
Cancer letters, 271(1), 1-12 (2008)
Nicole E Willmarth et al.
Breast cancer research : BCR, 6(5), R531-R539 (2004-08-21)
Cyclin E, a G1 cyclin essential for G1-S phase transition, is known to have a profound effect on tumorigenesis. Elevated levels of cyclin E have been associated with breast cancer, and chromosomal instability observed in breast cancer is suggested to
Harith Rajagopalan et al.
Nature, 428(6978), 77-81 (2004-03-06)
Aneuploidy, an abnormal chromosome number, has been recognized as a hallmark of human cancer for nearly a century; however, the mechanisms responsible for this abnormality have remained elusive. Here we report the identification of mutations in hCDC4 (also known as
Markus Welcker et al.
Current biology : CB, 14(20), 1852-1857 (2004-10-23)
The human tumor suppressor Fbw7/hCdc4 functions as a phosphoepitope-specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination of cyclin E , Notch , c-Jun and c-Myc . Fbw7 loss in cancer may thus have profound effects on
Masayoshi Yada et al.
The EMBO journal, 23(10), 2116-2125 (2004-04-23)
The F-box protein Skp2 mediates c-Myc ubiquitylation by binding to the MB2 domain. However, the turnover of c-Myc is largely dependent on phosphorylation of threonine-58 and serine-62 in MB1, residues that are often mutated in cancer. We now show that
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