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F2426

EZview™ Red 抗-FLAG® M2 亲和凝胶

clone M2

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Application:
IP, affinity chromatography
Citations:
204
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clone

M2, monoclonal

Quality Level

200

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

相关类别

General description

EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂，共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合，如细胞裂解物和组织，用于纯化标记蛋白，用于制备免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

Application

FLAG-和3xFLAG-标签融合蛋白的免疫沉淀（IP）。

洗脱-FLAG 多肽，甘氨酸，pH 3.5，3x FLAG 多肽

在我们的 FLAG® 应用门户了解更多产品详情。

Biochem/physiol Actions

适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

Physical form

1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

FLAG® 亲和凝胶，FLAG®标签，3xFLAG®标签，DYKDDDDK标签

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000441904
0000441904
0000432486
0000432486
0000269913

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Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Ting-Ting Qu et al.
BMC cancer, 18(1), 27-27 (2018-01-06)
Lin28B and its paralog Lin28A are small RNA binding proteins that have similar inhibitory effects, although they target separate steps in the maturation of let-7 miRNAs in mammalian cells. Because Lin28B participates in the promotion and development of tumors mostly
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实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀_蛋白纯化-默克生命科学

免疫沉淀（IP）可用于高效、高产量分离和纯化与FLAG®肽标签融合的蛋白质。此过程采用ANTI-FLAG® M2亲和凝胶进行，后者是一种与琼脂糖树脂共价结合的高度特异性单克隆抗体。

相关内容

蛋白表达_重组蛋白_蛋白质生物学-默克生命科学

默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间，以便进一步开展研究。

蛋白纯化

用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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全球贸易项目编号

货号GTIN
F2426-1ML04061838255358
F2426-500UL04061838248640
F2426-5X1ML04061838255365

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