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F2442

胎牛血清

Name: 胎牛血清
Brand: SIGMA

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

别名:

FBS, FCS, 血清

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关于此项目

UNSPSC Code:

12352207

eCl@ss:

32160409

NACRES:

NA.71

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

USA origin

Sterility:

sterile-filtered

Shipped in:

dry ice

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

胎牛血清（FBS）是一种由低、高分子量生物分子组成的复杂混合物，具有最佳的生长促进和抑制功能。FBS中含有如生长因子、蛋白质、维生素、微量元素、激素等多种分子。这些分子对于细胞的生长和维持至关重要。它是细胞培养基中的一种常见补剂。FBS来自于小牛血。它不含有纤维蛋白和凝血因子。FBS的热灭活可阻断补体系统。来源于凝结血的FBS是真核细胞，尤其是哺乳动物细胞培养中最广泛使用的未确定补充剂。它通常与在1950年代和1960年代开发的经典培养基一起使用。这些包括多种常用培养基，如Minimum Essential培养基（MEM）、Dulbecco′s改良Eagles培养基（DME，DMEM）、Iscove′s改良Eagles培养基（IMDM）、M199以及Roswell Park Memorial Institute培养基RPMI-1640。

Application

胎牛血清还可作为组分用于：

血管周围巨噬细胞样黑素细胞（PVM/M）生长培养基
合成输卵管液（SOF）
Eagle′s minimum essential培养基（EMEM）
neurobasal培养基
Hyclone McCoys 5 A培养基
Hanks培养基

Biochem/physiol Actions

FBS仍然是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子，并通过难以在无血清培养基（SFM）系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。

Analysis Note

内毒素和血红蛋白测试

检测了是否存在细菌、病毒和支原体
在无菌条件下用0.1微米膜过滤三次

Other Notes

对是否存在细菌、病毒、支原体、内毒素以及血红蛋白进行了检测。在无菌条件下用0.1微米膜过滤三次。

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.

Wie SM et al.

The Journal of Biological Chemistry, 289, 10900-10900 (2014)

Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium

Mosa FM, et al.

Ife Journal of Science, 4B, 3088-3093 (2015)

MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.

Grigoryev SA et al.

The Journal of Biological Chemistry, 274, 5626-5626 (1999)

Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.

van der Valk J et al.

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Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes.

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HIV-1 arose as the result of spillover of simian immunodeficiency viruses (SIVs) from great apes in Africa, namely from chimpanzees and gorillas. Chimpanzees and gorillas were, themselves, infected with SIV after virus spillover from African monkeys. During spillover events, SIV

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全球贸易项目编号

货号	GTIN
F2442-100ML	04061838338709
F2442-24X500ML	04061838255372
F2442-1L	04061837034589
F2442-50ML	04061838255396
F2442-500ML	04061838255389
F2442-6X500ML	04061838255402

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