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F2555

单克隆抗-FLAG®

clone SIG1-25, ascites fluid

别名:

抗-ddddk, 抗-dykddddk

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SIG1-25, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
133
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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SIG1-25, monoclonal

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

相关类别

General description

表位标签提供的方法可定位各种类型细胞中的基因产物、研究蛋白和蛋白复合物的拓扑结构、识别相关蛋白，并在没有蛋白特异性抗体时对新鉴定、丰度低或免疫原性低的蛋白进行表征。FLAG® 肽序列标签可以在N-端、前面有甲硫氨酸残基的N-端、C-端或在靶蛋白的内部位置进行。FLAG也可以与其他标签一起使用。短FLAG标签或序列及其高亲水性倾向于降低干扰蛋白表达、蛋白水解成熟、抗原性和功能的可能性。N-端FLAG® 肽序列含有独特的肠激酶切割位点，使其可以从纯化的融合蛋白中完全去除。已有报道，C-端FLAG® 肽的Cu2+离子，可从融合蛋白催化切割。在大鼠和小鼠Mg2+依赖性蛋白b-磷酸酶以及人类细胞和牛细胞酶中发现了一种序列模体，其八个氨基酸残基中有五个与FLAG®肽相同。兔抗-FLAG®抗体来自SIG1-25杂交瘤细胞。杂交瘤细胞是通过接种偶联KLH的FLAG®肽链序列的兔骨髓瘤细胞和脾细胞融合获得。

Immunogen

FLAG肽链序列DYKDDDK

Application

ANTI-FLAG®单克隆抗体（兔抗）适用：

  • 免疫荧光
  • 蛋白质印迹分析
  • 免疫细胞化学
  • 间接ELISA

更多产品信息，请访问我们的FLAG®应用页面。

Biochem/physiol Actions

这种单克隆抗体可识别FLAG序列N端

Physical form

以含有15mM叠氮化钠（作为防腐剂）的腹水形式提供。

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
动物来源生物产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000435608
0000435608
0000368976
0000223737
0000223737

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Recombinant lentivirus with enhanced expression of caudal-related homeobox protein 2 inhibits human colorectal cancer cell proliferation in vitro
He S, et al.
Molecular Medicine Reports, 12(2), 1838-1844 (2015)
Qungang Qi et al.
The Journal of biological chemistry, 287(37), 31482-31493 (2012-07-18)
Previous studies have demonstrated that Arabidopsis thaliana BBX32 (AtBBX32) represses light signaling in A. thaliana and that expression of AtBBX32 in soybean increases grain yield in multiple locations and multiyear field trials. The BBX32 protein is a member of the
Yury A Bochkov et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(17), 5485-5490 (2015-04-08)
Members of rhinovirus C (RV-C) species are more likely to cause wheezing illnesses and asthma exacerbations compared with other rhinoviruses. The cellular receptor for these viruses was heretofore unknown. We report here that expression of human cadherin-related family member 3
Increased protein stability of CDKN1C causes a gain-of-function phenotype in patients with IMAGe syndrome
Hamajima N, et al.
PLoS ONE, 8(9), e75137-e75137 (2013)
Jennifer J Banerjee et al.
eLife, 6 (2017-07-01)
Polarity is a shared feature of most cells. In epithelia, apical-basal polarity often coexists, and sometimes intersects with planar cell polarity (PCP), which orients cells in the epithelial plane. From a limited set of core building blocks (e.g. the Par
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Datasheet - F2555

全球贸易项目编号

货号GTIN
F2555-100UL04061833612972
F2555-200UL04061833612996

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