biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SIG1-25, monoclonal
technique(s)
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
isotype
IgG
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
表位标签提供的方法可定位各种类型细胞中的基因产物、研究蛋白和蛋白复合物的拓扑结构、识别相关蛋白,并在没有蛋白特异性抗体时对新鉴定、丰度低或免疫原性低的蛋白进行表征。FLAG® 肽序列标签可以在N-端、前面有甲硫氨酸残基的N-端、C-端或在靶蛋白的内部位置进行。FLAG也可以与其他标签一起使用。短FLAG标签或序列及其高亲水性倾向于降低干扰蛋白表达、蛋白水解成熟、抗原性和功能的可能性。N-端FLAG® 肽序列含有独特的肠激酶切割位点,使其可以从纯化的融合蛋白中完全去除。已有报道,C-端FLAG® 肽的Cu2+离子,可从融合蛋白催化切割。在大鼠和小鼠Mg2+依赖性蛋白b-磷酸酶以及人类细胞和牛细胞酶中发现了一种序列模体,其八个氨基酸残基中有五个与FLAG®肽相同。兔抗-FLAG®抗体来自SIG1-25杂交瘤细胞。杂交瘤细胞是通过接种偶联KLH的FLAG®肽链序列的兔骨髓瘤细胞和脾细胞融合获得。
Immunogen
FLAG肽链序列DYKDDDK
Application
Biochem/physiol Actions
这种单克隆抗体可识别FLAG序列N端
Physical form
以含有15mM叠氮化钠(作为防腐剂)的腹水形式提供。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)