产品名称
抗FLAG® M1单克隆抗体 小鼠抗, clone M1, purified immunoglobulin, buffered aqueous solution
biological source
(Bioreactor)
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M1, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot: 10 μg/mL
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
抗-FLAG® M1单克隆抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹和EIA分析。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
更多产品信息，请访问我们的FLAG®应用页面。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
更多产品信息，请访问我们的FLAG®应用页面。
Biochem/physiol Actions
当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合，因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性；该复合物在不存在钙离子的情况下解离。
General description
抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。
纯化方法-蛋白A
纯化方法-蛋白A
Immunogen
FLAG；肽序列 DYKDDDDK
Other Notes
Physical form
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
Preparation Note
将抗体稀释至 10ug/mL，置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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