产品名称
单克隆抗 FLAG ® M5 小鼠抗, clone M5, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M5, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot (chemiluminescent): 10 μg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
只有当 FLAG 肽位于甲硫氨酸前面的氨基末端时，才能与其结合。结合不依赖于 Ca2+。可用于检测哺乳动物细胞粗提物中细胞质表达的 Met-FLAG 融合蛋白，但不推荐用于在大肠杆菌中表达的融合蛋白。
小鼠生产的单克隆抗FLAG®M5抗体已被用于免疫印迹和免疫荧光。
General description
单克隆 ANTI-FLAG ® M5 是一种小鼠抗体，可与 N-末端 Met-FLAG 融合蛋白结合。可用于检测哺乳动物和果蝇细胞中表达的 N-末端 Met-FLAG 融合蛋白。
纯化方法-蛋白A
纯化方法-蛋白A
Immunogen
FLAG；肽序列 DYKDDDDK
Other Notes
抗体不具有钙依赖性。
Physical form
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
Preparation Note
用 Tris 缓冲盐水 (TBS) 将抗体稀释至 10 mg/mL：0.05 M Tris，0.15 M NaCl，pH 7.4。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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