biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
General description
FLAG M2-FITC被用于检测Flag融合蛋白。该单克隆抗体来自于小鼠,并与异硫氰酸荧光素(FITC)共价结合。该抗体可识别N末端、Met N末端和C末端的FLAG序列。结合不依赖于钙。F4049是一种亲和纯化的抗体,在大多数应用中可提高灵敏度。
Application
抗体适用于免疫荧光、免疫细胞化学、免疫组织化学、免疫印迹、流式细胞术和电子显微镜分析。该抗体特别适用于检测在小鼠宿主中表达的Flag融合蛋白,其中二抗可能引起交叉反应。
请访问我们的 FLAG® 应用 门户网站,了解更多产品详情。
请访问我们的 FLAG® 应用 门户网站,了解更多产品详情。
Physical form
溶于10 mM磷酸盐缓冲盐水,含有1% BSA和0.1%叠氮化钠,pH 7.4
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品?
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Pete Stavropoulos et al.
Journal of immunological methods, 362(1-2), 190-194 (2010-08-31)
The African trypanosome (Trypanosoma brucei) is transmitted by the bite of the tsetse vector to the mammalian bloodstream where it exists as a completely extracellular parasite. As a result of this exposure, the parasite elicits a robust immune response that
Xuechen Lv et al.
Protein & cell, 7(5), 325-337 (2016-04-18)
G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect
Jun-ichi Asaka et al.
Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)
Multidrug and toxin extrusion 1 (MATE1) has been isolated as an H(+)/organic cation antiporter located at the renal brush-border membranes. Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H(+)/organic cation
Jing Yi et al.
PloS one, 8(10), e78144-e78144 (2013-11-10)
LGR4 is an R-spondin receptor with strong positive effect on Wnt signaling. It plays a critical role in development as its ablation in the mouse led to total embryonic/neonatal lethality with profound defects in multiple organs. Haplotype insufficiency of LGR4
Emmanuelle Despras et al.
Nature communications, 7, 13326-13326 (2016-11-05)
Translesion polymerase eta (polη) was characterized for its ability to replicate ultraviolet-induced DNA lesions that stall replicative polymerases, a process promoted by Rad18-dependent PCNA mono-ubiquitination. Recent findings have shown that polη also acts at intrinsically difficult to replicate sequences. However
商品
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持