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Merck
CN

F4135

胎牛血清

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

别名:

HI FBS, HI FCS, HI 血清, FBS, FCS, 血清

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关于此项目

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

胎牛血清(FBS)一直是一种常用的培养基补充剂,因为它在细胞培养中提供了广泛的功能。FBS提供营养物、生长和附着因子,并有助于增殖。它还保护细胞免于氧化损伤和细胞凋亡。FBS阻断活性氧的生成,否则在无血清培养基(SFM)系统中不会消除这种活性氧。

Application

来自美国的FBS被广泛用于细胞培养应用中。FBS可为培养的细胞提供了多种未确定的生长促进和存活增强因子。
胎牛血清已用于:
  • 作为Dulbecco′s Modified Eagles Media (DME, DMEM)、 Eagle′s Minimum Essential Medium (EMEM)、 Roswell Park Memorial Institute 培养基 (RPMI-1640)、 α-改良 eagle培养基(αMEM) (MEM)和 Leibovitz L-15培养基的成分
  • 作为处理脑干切片的封闭介质的一种成分
  • 用于改善真皮外植体的附着

Preparation Note

在56°C加热30分钟制备。
该产品是在56°C加热30分钟而制备的。将血清加热至56°C 30分钟可灭活补体系统以进行免疫测定。热激活还会使培养物中其他未确定的细胞生长抑制剂失活。

Analysis Note

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

监管及禁止进口产品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)

商品

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。

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实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

相关内容

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全球贸易项目编号

货号GTIN
F4135-100ML04061838255419
F4135-24X500ML04061833617533
F4135-1L04061837034718
F4135-6X500ML04061838255433
F4135-500ML04061838255426

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