F4383
7-氟苯并呋喃-4-磺酸 铵盐
≥98%
别名:
4-氟-7-磺酸苯并呋咱 铵盐, 7-氟苯呋咱-4-硫酸铵盐, 7-氟苯并-2,1,3-噁二唑-4-磺酸 铵盐, SBD-F
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关于此项目
线性分子式:
C6H3FN2O4S · NH3
化学文摘社编号:
分子量:
235.19
Beilstein:
5199564
MDL编号:
UNSPSC代码:
12352106
PubChem化学物质编号:
NACRES:
NA.32
一般描述
7-氟苯呋嗪-4-磺酸铵盐，又称SBD-F，是一种低分子量敏感荧光探针。SBD-F与巯基发射反应（二硫化物不与SBD-F反应，必须首先还原为硫醇，如三丁基膦），产生高荧光化合物。 硫醇与SBD-F的反应速率随pH升高而逐渐增加。在pH值2-12时观察到高荧光强度。 SBD-F的激发范围为380-385nm，发射范围为510-515nm。
应用
SBD-F适用于皮摩尔级别生物硫醇的HPLC测定。SBD-F已用于通过HPLC方法测量总血浆和血清同型半胱氨酸水平。它也适用于通过串联柱高效液相色谱法和等溶剂体系测定金属硫蛋白。 SBD-F已用于确定蛋白质中半胱氨酸残基二硫键的位置以及检测含半胱氨酸的多肽。
硫醇的荧光探针，HPLC 中生物硫醇的柱前标记物
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
J Tanaka et al.
Journal of biochemistry, 127(4), 597-601 (2000-03-31)
Pseudomonas sp. 109 produces a unique lipase (LipL) which efficiently catalyzes intramolecular transesterification of omega-hydroxyesters to form macrocyclic lactones. The production of the enzymatically active LipL requires a specific molecular chaperon (LimL protein) together with a low-M(r) lipase-activation-factor (LAF) of
J B Ubbink
Seminars in thrombosis and hemostasis, 26(3), 233-241 (2000-09-30)
Analytical methods to measure plasma total homocyst(e)ine (tHcy) concentrations are reviewed. High-pressure liquid chromatography (HPLC) with fluorometric detection is the most widely used method to determine plasma tHcy concentrations. Both monobromobimane and ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F) are popular thiol-specific fluorogenic agents
I Daskalakis et al.
Biomedical chromatography : BMC, 10(5), 205-212 (1996-09-01)
A sensitive HPLC-fluorescence method for determining total endogenous plasma homocysteine (Hcy), cysteine (Cys) and cysteinylglycine (Cys-Gly) following derivatization with ammonium 7-fluoro 2,1,3-benzoxadiazole-4-sulphonate (SBD-F) is described. Quantitation utilizes an internal standard, 2-mercaptoethylamine. The derivatization procedure has been optimized for concentration of
S Miyairi et al.
Analytical biochemistry, 258(2), 168-175 (1998-05-08)
Metallothionein (MT) is a low-molecular-weight protein which plays a role in detoxification of heavy metals and protection against oxidative stress. A sensitive and convenient determination method for MT is necessary to clarify its physiological roles. High-performance liquid chromatography (HPLC) with
Fluorometric assay of thiols with fluorobenzoxadiazoles.
K Imai et al.
Methods in enzymology, 143, 67-75 (1987-01-01)
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