F4383
7-氟苯并呋喃-4-磺酸 铵盐
≥98%
别名:
4-氟-7-磺酸苯并呋咱 铵盐, 7-氟苯呋咱-4-硫酸铵盐, 7-氟苯并-2,1,3-噁二唑-4-磺酸 铵盐, SBD-F
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关于此项目
线性分子式:
C6H3FN2O4S · NH3
化学文摘社编号:
分子量:
235.19
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5199564
产品名称
7-氟苯并呋喃-4-磺酸 铵盐, ≥98%
InChI
1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
SMILES string
N.OS(=O)(=O)c1ccc(F)c2nonc12
InChI key
JXLHNMVSKXFWAO-UHFFFAOYSA-N
assay
≥98%
storage condition
protect from light
fluorescence
λex 380 nm; λem 515 nm
storage temp.
−20°C
Quality Level
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Application
SBD-F适用于皮摩尔级别生物硫醇的HPLC测定。SBD-F已用于通过HPLC方法测量总血浆和血清同型半胱氨酸水平。它也适用于通过串联柱高效液相色谱法和等溶剂体系测定金属硫蛋白。 SBD-F已用于确定蛋白质中半胱氨酸残基二硫键的位置以及检测含半胱氨酸的多肽。
硫醇的荧光探针，HPLC 中生物硫醇的柱前标记物
General description
7-氟苯呋嗪-4-磺酸铵盐，又称SBD-F，是一种低分子量敏感荧光探针。SBD-F与巯基发射反应（二硫化物不与SBD-F反应，必须首先还原为硫醇，如三丁基膦），产生高荧光化合物。 硫醇与SBD-F的反应速率随pH升高而逐渐增加。在pH值2-12时观察到高荧光强度。 SBD-F的激发范围为380-385nm，发射范围为510-515nm。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
P E Cornwell et al.
Journal of chromatography, 617(1), 136-139 (1993-07-23)
A modification of a previously published method for analysis of total homocysteine in human serum is presented. The modification was implemented to allow use of a different derivatizing agent (i.e., 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonamide) which reacts much faster than the original derivatizing reagent
J B Ubbink et al.
Clinical chemistry, 45(5), 670-675 (1999-05-01)
Various methods are available to measure plasma total homocyst(e)ine (tHcy) concentrations, but whether plasma tHcy assays may be used interchangeably is not known. Results from three different methods [HPLC with fluorescence detection, enzyme immunoassay (EIA), and fluorescence polarization immunoassay (FPIA)]
Fluorometric assay of thiols with fluorobenzoxadiazoles.
K Imai et al.
Methods in enzymology, 143, 67-75 (1987-01-01)
S Miyairi et al.
Analytical biochemistry, 258(2), 168-175 (1998-05-08)
Metallothionein (MT) is a low-molecular-weight protein which plays a role in detoxification of heavy metals and protection against oxidative stress. A sensitive and convenient determination method for MT is necessary to clarify its physiological roles. High-performance liquid chromatography (HPLC) with
I Daskalakis et al.
Biomedical chromatography : BMC, 10(5), 205-212 (1996-09-01)
A sensitive HPLC-fluorescence method for determining total endogenous plasma homocysteine (Hcy), cysteine (Cys) and cysteinylglycine (Cys-Gly) following derivatization with ammonium 7-fluoro 2,1,3-benzoxadiazole-4-sulphonate (SBD-F) is described. Quantitation utilizes an internal standard, 2-mercaptoethylamine. The derivatization procedure has been optimized for concentration of
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