biological source
bovine kidney
form
ammonium sulfate suspension
specific activity
≥2.0 units/mg protein (biuret)
mol wt
210-220 kDa (Subunits: 55-60 kDa)
foreign activity
α-mannosidase and β-galactosidase ≤0.1%, β-N-acetylglucosaminidase ≤0.2%
shipped in
wet ice
storage temp.
2-8°C
General description
α-L-岩藻糖苷酶是脂质体酶,属于糖基水解酶类。
Application
α-L-岩藻糖苷酶(来源牛肾)用于:
- 研究其改变原代细胞转导的能力
- 研究细胞表面聚糖中岩藻糖残基在黏附中的作用
- 研究其在受精过程中的作用。
Biochem/physiol Actions
α-L-岩藻糖苷酶降解含岩藻糖的岩藻糖缀合物,破坏低聚糖和糖缀合物中的岩藻糖苷键。 可作为肝癌和大肠癌的肿瘤标志物。α-L-岩藻糖苷酶表现出转糖基化特性。
Physical form
悬浮于 NH4)2SO4,10 mM NaH2PO4 10mM 柠檬酸盐,pH 6.0
Other Notes
一个单元在 pH5.5,25℃ 下,每分钟将 1.0 μmole 对硝基苯基 α-L-岩藻糖苷水解成对硝基苯酚和 L-岩藻糖。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Directed evolution of the alpha-L-fucosidase from Thermotoga maritima into an alpha-L-transfucosidase
Osanjo G, et al.
Biochemistry, 46(4), 1022-1033 (2007)
Novel alpha-l-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides
Lezyk M, et al.
PLoS ONE, 11(1), e0147438-e0147438 (2016)
Human alpha-L-fucosidase-1 attenuates the invasive properties of thyroid cancer
Vecchio G, et al.
Testing, 8(16), 27075-27075 (2017)
alpha-l-Fucosidase enhances capacitation-associated events in porcine spermatozoa
Romero-Aguirregomezcorta J, et al.
The Veterinary Journal, 203(1), 109-114 (2015)
Alpha-L-Fucosidase Serves as a Prognostic Indicator for Intrahepatic Cholangiocarcinoma and Inhibits Its Invasion Capacity
Shuang Z, et al.
BioMed Research International, 2018 (2018)
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
Datasheet
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