F6636
营养混合物 F-12 Ham
Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture
别名:
Ham’s F-12
产品名称
营养混合物 F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
表单
powder
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
组分
phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L
运输
ambient
储存温度
2-8°C
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应用
库恩对哈姆氏 F-12 的改造是为培养通过病毒融合产生的杂交细胞而开发的。修饰包括使氨基酸和丙酮酸加倍，并包括抗坏血酸。盐浓度也发生了改变。配方中含有 0.863 mg/L 硫酸锌，这可能使其不适合培养小鼠 L -细胞。
制备说明
按照每升培养基11.5克粉末配制。
补充2.68 g/L碳酸氢钠。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源培养基
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Feng Liang et al.
SLAS technology, 22(3), 315-324 (2017-04-06)
Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). A large number of nearly 2000 reported mutations, including the premature termination codon (PTC) mutations, urgently require new
Xiaojiao Xue et al.
Human molecular genetics, 26(16), 3116-3129 (2017-06-03)
In-frame premature termination codons (PTCs) account for ∼11% of all disease-associated mutations. PTC suppression therapy utilizes small molecules that suppress translation termination at a PTC to restore synthesis of a full-length protein. PTC suppression is mediated by the base pairing
Robert Rauscher et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(4) (2021-01-21)
Epistasis refers to the dependence of a mutation on other mutation(s) and the genetic context in general. In the context of human disorders, epistasis complicates the spectrum of disease symptoms and has been proposed as a major contributor to variations
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target
Kenneth A Giuliano et al.
SLAS discovery : advancing life sciences R & D, 23(2), 111-121 (2017-09-13)
Cystic fibrosis (CF) is a lethal genetic disorder caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Despite recent groundbreaking approval of genotype-specific small-molecule drugs, a significant portion of CF patients still lack effective therapeutic options that
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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