跳转至内容
Merck
CN

F8435

Sigma-Aldrich

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

登录查看公司和协议定价

选择尺寸

关于此项目

化学文摘社编号:
83534-39-8
EC 号:
3.5.1.52 (BRENDA, IUBMB)
MDL编号:
MFCD18839214
UNSPSC代码:
12352204
NACRES:
NA.54

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌, lyophilized powder, recombinant, expressed in E. coli

重组

expressed in E. coli

质量水平

200

偶联物

(N-linked)

等级

Proteomics Grade

表单

lyophilized powder

比活

≥1,000 U/mg

保质期

≥1 weeks at 2‑8 °C (for reconstituted solution)
≥1 yr at -20 °C

分子量

~36 kDa

运输

wet ice

储存温度

−20°C

正在寻找类似产品? 访问 产品对比指南

相关类别

应用

用于使蛋白质去糖基化。
高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

生化/生理作用

从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

包装

在一项调查他汀类药物对p-糖蛋白的双重影响及其对人神经母细胞瘤细胞中阿霉素细胞毒性影响的研究中，PNGase F已被用于P-糖蛋白的去糖基化。它在一项研究中的定量免疫印迹分析前被用于处理纯化的蛋白，小鼠视锥紫外线（MUV）色素它已被用于对N-连接的糖蛋白进行糖基化。高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

其他说明

一个单元将在37℃、pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000432197
0000432197
0000405902
0000405901
0000405901

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Maria Nordgren et al.
Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
Tobias Bierig et al.
Frontiers in bioengineering and biotechnology, 8, 618615-618615 (2021-01-08)
2019-nCoV is the causative agent of the serious, still ongoing, worldwide coronavirus disease (COVID-19) pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays, and to the general understanding of virus
Clarissa Strieder-Barboza et al.
Scientific reports, 9(1), 19748-19748 (2019-12-26)
The adipose tissue extracellular matrix (ECM) regulates adipocyte cellular metabolism and is altered in obesity and type 2 diabetes, but mechanisms underlying ECM-adipocyte metabolic crosstalk are poorly defined. Advanced glycation end-product (AGE) formation is increased in diabetes. AGE alter tissue
Evelyn Sieczkowski et al.
International journal of cancer, 126(9), 2025-2035 (2009-09-10)
The development of multidrug resistance (MDR) is a major problem during cancer treatment. Drug efflux via ATP-binding cassette (ABC) transporters is the main mechanism responsible for resistance to chemotherapeutics. We have recently observed that statins enhance susceptibility to doxorubicin-induced apoptosis
T H Plummer et al.
The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
查看所有相关文献

商品

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Improving Glycoform Coverage for Mucin Preparations

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

实验方案

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持