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Merck
CN

F8435

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

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关于此项目

化学文摘社编号:
83534-39-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC 号:
3.5.1.52 (BRENDA, IUBMB)
MDL number:
MFCD18839214
Specific activity:
≥1,000 U/mg
Recombinant:
expressed in E. coli
Shelf life:
≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

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产品名称

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌, lyophilized powder, recombinant, expressed in E. coli

recombinant

expressed in E. coli

conjugate

(N-linked)

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥1,000 U/mg

shelf life

≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

mol wt

~36 kDa

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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相关类别

Application

用于使蛋白质去糖基化。
高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

Biochem/physiol Actions

从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

Packaging

在一项调查他汀类药物对p-糖蛋白的双重影响及其对人神经母细胞瘤细胞中阿霉素细胞毒性影响的研究中，PNGase F已被用于P-糖蛋白的去糖基化。它在一项研究中的定量免疫印迹分析前被用于处理纯化的蛋白，小鼠视锥紫外线（MUV）色素它已被用于对N-连接的糖蛋白进行糖基化。高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

Other Notes

一个单元将在37℃、pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000432197
0000432197
0000405901
0000405902
0000405901

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Maria Nordgren et al.
Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
Clarissa Strieder-Barboza et al.
Scientific reports, 9(1), 19748-19748 (2019-12-26)
The adipose tissue extracellular matrix (ECM) regulates adipocyte cellular metabolism and is altered in obesity and type 2 diabetes, but mechanisms underlying ECM-adipocyte metabolic crosstalk are poorly defined. Advanced glycation end-product (AGE) formation is increased in diabetes. AGE alter tissue
Tobias Bierig et al.
Frontiers in bioengineering and biotechnology, 8, 618615-618615 (2021-01-08)
2019-nCoV is the causative agent of the serious, still ongoing, worldwide coronavirus disease (COVID-19) pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays, and to the general understanding of virus
Evelyn Sieczkowski et al.
International journal of cancer, 126(9), 2025-2035 (2009-09-10)
The development of multidrug resistance (MDR) is a major problem during cancer treatment. Drug efflux via ATP-binding cassette (ABC) transporters is the main mechanism responsible for resistance to chemotherapeutics. We have recently observed that statins enhance susceptibility to doxorubicin-induced apoptosis
T H Plummer et al.
The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
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