biological source
bovine fetus
Quality Segment
sterility
γ-irradiated, sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤0.18 mg/mL
origin
Australia origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
胎牛血清已用于Roswell Park memorial institute培养基-1640 (RPMI-1640),以培养骨髓瘤细胞系,RPMI-8226和U-266。它也被用作Dulbecco改性Eagle培养基(DMEM)的补充物,用于培养人脐静脉内皮细胞(HUVEC)和293 T细胞。
Biochem/physiol Actions
胎牛血清(FBS)由于其高营养成分,是用于补充细胞培养基的最常见的血清。FBS虽然蛋白质含量低,但对促进和维持脊椎动物、哺乳动物和昆虫细胞的生长很有效。它可以作为胚胎干细胞培养的补充物。FBS也可用于制备喂料层和条件介质。
Analysis Note
- 内毒素和血红蛋白测试
Other Notes
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable