F9679
Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit
1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-FAS, Anti-FASN
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 270 kDa
species reactivity
mouse, rat, human
concentration
1 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using lysates of Rat1 cells, western blot: 1-2 μg/mL using lysates of human HEK 293-T cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FASN(2194)
mouse ... Fasn(14104)
rat ... Fasn(50671)
General description
Fatty acid synthase (FAS, FASN) is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).
Immunogen
synthetic peptide corresponding to amino acids 2497-2511 of human fatty acid synthase, conjugated to KLH via an N-terminal added lysine residue. The corresponding sequence is conserved in human, rat, and mouse.
Application
Anti-Fatty Acid Synthase (C-Terminal) antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of Rat1 cells and 1-2μg/mL using lysates of human HEK 293-T cells.
Biochem/physiol Actions
FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
C Rufo et al.
The Journal of biological chemistry, 276(24), 21969-21975 (2001-03-30)
Refeeding carbohydrate to fasted rats induces the transcription of genes encoding enzymes of fatty acid biosynthesis, e.g. fatty-acid synthase (FAS). Part of this transcriptional induction is mediated by insulin. An insulin response element has been described for the fatty-acid synthase
Francisco J Asturias et al.
Nature structural & molecular biology, 12(3), 225-232 (2005-02-16)
De novo synthesis of fatty acids in the cytosol of animal cells is carried out by the multifunctional, homodimeric fatty acid synthase (FAS). Cryo-EM analysis of single FAS particles imaged under conditions that limit conformational variability, combined with gold labeling
Sucharita Bandyopadhyay et al.
Oncogene, 24(34), 5389-5395 (2005-05-18)
Fatty acid synthase (FAS), a key enzyme of the fatty acid biosynthetic pathway, has been shown to be overexpressed in various types of human cancer and is, therefore, considered to be an attractive target for anticancer therapy. However, the exact
Ellen De Schrijver et al.
Cancer research, 63(13), 3799-3804 (2003-07-04)
Fatty acid synthase (FASE), a key enzyme in the biosynthesis of fatty acids, is markedly overexpressed in many human epithelial cancers, rendering it an interesting target for antineoplastic therapy. Here, using the potent and highly sequence-specific mechanism of RNA interference
Fatty acid synthase, a proficient multifunctional enzyme.
S J Wakil
Biochemistry, 28(11), 4523-4530 (1989-05-30)
商品
Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.
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