产品名称
萤火虫尾部提取物, for ATP determination
form
solution
packaging
pkg of 250 mg dried lanterns
technique(s)
toxicology assay: suitable
storage temp.
−20°C
Quality Level
Application
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
Biochem/physiol Actions
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
General description
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
Other Notes
Crude luciferin and luciferase
Preparation Note
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源生物产品
此项目有
Distribution patterns of organic pollutants and microbial processes in marine sediments across a gradient of anthropogenic impact
Zoppini A, et al.
Environmental Pollution (Barking, Essex : 1987), 242, 1860-1870 (2018)
Concentrations and uptake of dissolved organic phosphorus compounds in the Baltic Sea
Nausch M, et al.
Frontiers in Marine Science, 5(386) (2018)
Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against beta-amyloid-induced alterations in red blood cells
Carelli-Alinovi C, et al.
Biochimie, 121, 52-59 (2016)
Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions
Marques SM, et al.
IUBMB Life, 61(1), 6-17 (2009)
Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin
Gomi K and Kajiyama N
The Journal of Biological Chemistry, 276(39), 36508-36513 (2001)
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