一般描述
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
应用
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
生化/生理作用
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
制备说明
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.
其他说明
Crude luciferin and luciferase
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源生物产品
此项目有
Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against beta-amyloid-induced alterations in red blood cells
Carelli-Alinovi C, et al.
Biochimie, 121, 52-59 (2016)
Concentrations and uptake of dissolved organic phosphorus compounds in the Baltic Sea
Nausch M, et al.
Frontiers in Marine Science, 5(386) (2018)
Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions
Marques SM, et al.
IUBMB Life, 61(1), 6-17 (2009)
Distribution patterns of organic pollutants and microbial processes in marine sediments across a gradient of anthropogenic impact
Zoppini A, et al.
Environmental Pollution (Barking, Essex : 1987), 242, 1860-1870 (2018)
Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin
Gomi K and Kajiyama N
The Journal of Biological Chemistry, 276(39), 36508-36513 (2001)
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