Merck
CN

G0282

Sigma-Aldrich

粒细胞巨噬细胞集落刺激因子 来源于小鼠

GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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别名:
GM-CSF
MDL编号:
NACRES:
NA.77

生物来源

mouse

质量水平

重组

expressed in E. coli

检测方案

≥97% (SDS-PAGE)

形式

lyophilized powder

效能

≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)

质量

endotoxin tested

分子量

~15 kDa (125 amino acids including N-terminal methionine)

包装

pkg of 5 and 50 μg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

≤1 EU/mg

颜色

white

溶解性

water: soluble, clear, colorless

UniProt登记号

储存温度

−20°C

基因信息

生化/生理作用

粒细胞-巨噬细胞集落刺激因子(GM-CSF)是粒细胞、巨噬细胞和嗜酸性粒细胞系的生长和分化因子。GM-CSF 在极低浓度下刺激多能祖细胞集落形成,是造血祖细胞直至成熟的所有分裂中必不可少的存活和增殖因子。它也刺激一些上皮细胞和破骨细胞的生长。GM-CSF 由多种细胞类型(单核细胞、内皮细胞、T 细胞、成纤维细胞、有丝分裂原刺激的 B 细胞和 LPS 刺激的巨噬细胞)产生。GM-CSF 是一种单链糖蛋白,含有 128 个氨基酸,具有保守的二硫键。人和鼠 GM-CSF 的序列同源性约为 54%,生物活性无交叉反应。

外形

从 10 mM 乙酸 + 250 μg BSA 中冻干。

分析说明

使用小鼠 FDP-1 细胞在培养物中检测了小鼠 GM-CSF 的 EC50 活性。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
含少量动物源组分生物产品

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R C Skoda
Journal of receptor and signal transduction research, 19(1-4), 741-772 (1999-03-11)
The helical cytokines constitute a family of proteins with a common three-dimensional structure. They exert a wide variety of biological effects with a preference for the hematopoietic system. The effects of helical cytokines are mediated by cell surface receptors, which
A Kelso
Immunology and cell biology, 76(4), 300-317 (1998-09-02)
Cytokines participate in the induction and effector phases of all immune and inflammatory responses. They are therefore obvious tools and targets for strategies designed to promote, inhibit or redirect these responses. However, the complexity of the cytokine network has hindered
M A Guthridge et al.
Stem cells (Dayton, Ohio), 16(5), 301-313 (1998-10-10)
The process of ligand binding leading to receptor activation is an ordered and sequential one. High-affinity binding of GM-CSF, interleukin 3 (IL-3), and IL-5 to their receptors induces a number of key events at the cell surface and within the
Bin Ji et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(47), 12255-12267 (2008-11-21)
We demonstrate the significance of peripheral benzodiazepine receptor (PBR) imaging in living mouse models of Alzheimer's disease (AD) as biomarkers and functional signatures of glial activation. By radiochemically and immunohistochemically analyzing murine models of the two pathological hallmarks of AD
M H Heim
Journal of receptor and signal transduction research, 19(1-4), 75-120 (1999-03-11)
The Jak-STAT pathway was originally discovered through the study of interferon induced intracellular signal transduction. Meanwhile, a large number of cytokines, hormones and growth factors have been found to activate Jaks and STATs. Jaks (Janus Kinases) are a unique class

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