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Merck
CN

G0762

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于罗曼蜗牛

Type H-3AF, aqueous solution, ≥60,000 units/mL

别名:

β-葡萄糖醛酸苷酶

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化学文摘社编号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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生物来源

Helix pomatia

质量水平

类型

Type H-3AF

表单

aqueous solution

比活

≥60,000 units/mL

杂质

≤1,000 unit/mL Sulfatase

运输

wet ice

储存温度

2-8°C

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应用

比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡

生化/生理作用

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解

外形

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

分析说明

来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。

其他说明

在pH 5.0、温度37°C条件下,一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞(30分钟实验)。
硫酸酯酶单元定义:一单位的硫酸酯酶每小时,pH5.0,37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
A further processing of Type H-3 to remove agglutinin.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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W Lechner et al.
Acta endocrinologica, 109(2), 266-268 (1985-06-01)
Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.
Kenneth K C Bramwell et al.
The Journal of clinical investigation, 124(1), 311-320 (2013-12-18)
Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most prevalent arthropod-borne illness in the United States and remains a clinical and social challenge. The spectrum of disease severity among infected patients suggests that host genetics contribute to pathogenic
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system
Tracey B Tucker Zhou et al.
The Journal of biological chemistry, 288(51), 36302-36311 (2013-11-13)
Klotho (KL) is an age-regulating protein named after the Greek goddess who spins the thread of life. Mice deficient in KL are normal throughout development, but rapidly degenerate and display a variety of aging-associated abnormalities that eventually lead to decreased
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded

商品

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

实验方案

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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