Merck
CN

G0762

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于罗曼蜗牛

Type H-3AF, aqueous solution, ≥60,000 units/mL

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别名:
β-葡萄糖醛酸苷酶
CAS号:
MDL编号:
NACRES:
NA.54

生物来源

Helix pomatia

质量水平

类型

Type H-3AF

形式

aqueous solution

比活

≥60,000 units/mL

杂质

≤1,000 unit/mL Sulfatase

运输

wet ice

储存温度

2-8°C

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应用

比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡

生化/生理作用

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解

质量

来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。

联系

A further processing of Type H-3 to remove agglutinin.

单位定义

在pH 5.0、温度37°C条件下,一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞(30分钟实验)。
硫酸酯酶单元定义:一单位的硫酸酯酶每小时,pH5.0,37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。

外形

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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商品

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

实验方案

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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