G0799
β-葡萄糖醛酸酶 来源于大肠杆菌
>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution
别名:
β-D-葡萄糖苷酸葡萄糖醛酸水解酶
重组
expressed in E. coli
质量水平
表单
aqueous glycerol solution
比活
>20,000,000 units/g protein
分子量
74 kDa
浓度
>1 mg/mL
运输
wet ice
储存温度
−20°C
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一般描述
β-葡萄糖醛酸酶是属于糖苷酶家族的四聚体，分子量为 29 kDa。它由 74 kDa 相同的单体组成。该酶的 pI 和最适 pH 值分别为 4.8 和 6-7。
外形
β葡萄糖醛酸酶在GUS测定中可用作报告基因以监测基因的表达。
含50%甘油的高纯度溶液
其他说明
一个Sigma或改良的Fishman单位将在37℃，pH6.8（30分钟测定）下每小时从酚酞葡糖苷酸中释放1.0μg酚酞。
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
D H Kim et al.
Biological & pharmaceutical bulletin, 18(9), 1184-1188 (1995-09-01)
beta-Glucuronidase was purified 360-fold from Escherichia coli HGU-3, an human intestinal bacterium. The specific activity of the purified enzyme was 17.78 units/mg protein. The enzyme (M.W. 290000) is composed of four subunits (M.W. 72000) with a pI and optimal pH
E M Schmelz et al.
Cancer research, 59(22), 5768-5772 (1999-12-03)
Dietary sphingolipids inhibit chemically induced colon cancer in mice. The most likely mediators of this effect are the metabolites ceramide (Cer) and sphingosine, which induce growth arrest and apoptosis in transformed cells. Sphingolipids are digested in both the upper and
Fei Song et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 67(11-12), 611-619 (2013-02-19)
Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
实验方案
为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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