质量水平
技术
protein array: suitable
基质活性基团
phase
溶胀
1 g swells to 2-3 mL
应用
life science and biopharma
相容性
Cytiva
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一般描述
Sephadex G-10葡聚糖凝胶是常见的凝胶过滤树脂，专门设计用于多肽和生物小分子的脱盐和缓冲液置换。该型号具有Sephadex中最小的排阻极限，令其在这些应用中十分有效。Sephadex是葡聚糖与环氧氯丙烷交联制备而成的凝胶过滤树脂。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分级范围也不同。Sephadex G-10属于G型，G型Sephadex的型号包括从G-10（用于小分子）到G-75（用于大分子）的5种型号。
应用
Sephadex® G-10可作为体积排阻材料用于将标记的N-聚糖与多余的染料分离。还可用于脱盐以降低盐浓度用于阴离子交换(AEx)和阳离子交换色谱(CEx)。
Sephadex® G-10已被用于将膜蛋白重建为巨大的蛋白脂质体。
生化/生理作用
Sephadex G-10是凝胶过滤介质，用于凝胶过滤色谱和蛋白质色谱。应用广泛，已被用于癌症研究，并可用于测定自来水、河水和绿茶等多种样品中的氟化物含量。
特点和优势
- 一步完成脱盐、除污染和置换新缓冲液。
- 适合用于缓冲液置换和生物样品纯化。
- 适合分离分子量超过700的多肽、小分子蛋白和多糖。
- 高效去除盐、染料和放射性标记。
- 具有Sephadex G型中最低的700分子量排阻极限
法律信息
Sephadex is a registered trademark of Cytiva
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jinlan Zhang et al.
Journal of food protection, 72(12), 2524-2529 (2009-12-17)
Pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31-1 from the traditional Chinese fermented Xuan-Wei ham, was successfully purified by the pH-mediated cell adsorption-desorption method and then purified by gel chromatography with Sephadex G-10. The purification resulted in a
N-glycan analysis by CGE-LIF: profiling influenza A virus hemagglutinin N-glycosylation during vaccine production
Schwarzer J, et al.
Electrophoresis (2008)
Kaushal Rege et al.
Nature protocols, 5(3), 408-417 (2010-03-06)
Methods development in chromatography is a time-consuming, trial-and-error process that requires laborious experimentation. We describe a high-throughput screening (HTS) protocol for the rapid identification of chromatographic steps for protein purification from cell-free expression broths. Broths containing the protein are loaded
H Han et al.
Nucleic acids research, 22(14), 2837-2844 (1994-07-25)
Different helical conformations of DNA (D), RNA (R), and DNA.RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA.Fe to a single nucleotide on RNA as well as DNA
Miniaturized parallel screens to identify chromatographic steps required for recombinant protein purification
Rege K and Heng M
Nature Protocols (2010)
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