G1163
O-糖苷酶 来源于肺炎链球菌
recombinant, expressed in E. coli, buffered aqueous solution
别名:
α-内切乙酰氨基半乳糖苷酶, O-糖酵素
重组
expressed in E. coli
质量水平
偶联物
(O-linked)
表单
buffered aqueous solution
分子量
180 kDa
浓度
≥800 units/mL
运输
wet ice
储存温度
2-8°C
生化/生理作用
从糖蛋白上释放未取代的Ser-和Thr-连接β-Gal-(1→3)-α-GalNAc（核心1型O-聚糖)。 用唾液酸、乳糖胺（半乳糖-N-乙酰氨基葡萄糖）或岩藻糖取代的二糖核心将阻止水解并防止寡糖从蛋白质中释放出来。在使用O-糖苷酶切割之前，可能需要使用糖酵解酶进行预处理以从O-聚糖中去除取代糖。
包装
提供5×反应缓冲液，250mM NaH2PO4，pH 5.0。
外形
溶于50 mM磷酸钾，pH 7.5
分析说明
筛查是否存在：β-半乳糖苷酶、α-甘露糖苷酶、β-己糖胺酶、α-岩藻糖苷酶、神经氨酸酶和蛋白酶。有关特定批次的信息，请参阅分析证书。
其他说明
在37 °C、pH 6.5下，一单位在每分钟内可水解1 μmole的对硝基苯基半乳糖N-生物苷（β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2）。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
K Sonoda et al.
Cancer, 77(8), 1501-1509 (1996-04-15)
A large number of monoclonal antibodies (MoAbs) against human tumor cells have been generated and it has been shown that these MoAbs are useful tools in the diagnosis and treatment of cancer patients, as well as in the basic investigation
Ryoko Akai et al.
The Analyst, 128(5), 440-446 (2003-06-07)
O-Glycans (mucin type oligosaccharides) are ubiquitously found in various glycoproteins such as mucin-type glycoproteins on the surface of various digestive organs. In the present paper, we propose a method for detecting O-glycanase which catalyzes the hydrolysis of the O-glycan linkage
Roudabeh J Jamasbi et al.
Hybridoma and hybridomics, 22(6), 367-376 (2003-12-20)
A mouse monoclonal antibody (MAb-9) produced by immunization with a human esophageal carcinoma cell line, TE-2 (derived from undifferentiated squamous cell carcinoma) reacted specifically with about 30% of esophageal carcinoma cell lines and tissue sections from clinical samples. MAb-9 showed
Nelia A Tobey et al.
Digestive diseases and sciences, 55(7), 1856-1865 (2010-05-27)
The structures that contribute to shunt resistance (Rs) in esophageal epithelium are incompletely understood, with 35-40% of Rs known to be calcium-dependent, reflecting the role of e-cadherin. Two calcium-independent candidates for the remaining approximately 60% of Rs have been identified:
I Brockhausen
Biochimica et biophysica acta, 1473(1), 67-95 (1999-12-02)
Glycoproteins with O-glycosidically linked carbohydrate chains of complex structures and functions are found in secretions and on the cell surfaces of cancer cells. The structures of O-glycans are often unusual or abnormal in cancer, and greatly contribute to the phenotype
商品
Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.
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