Merck
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文件

G2025

Sigma-Aldrich

甘油

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)

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别名:
1,2,3-丙三醇, 甘油
线性分子式:
HOCH2CH(OH)CH2OH
CAS号:
56-81-5
分子量:
92.09
Beilstein:
635685
EC 号:
200-289-5
MDL编号:
MFCD00004722
PubChem化学物质编号:
24895082
NACRES:
NA.21

蒸汽密度

3.1 (vs air)

质量水平

300

蒸汽压

<1 mmHg ( 20 °C)

产品线

BioReagent

检测方案

≥99% (GC)

形式

viscous liquid

自燃温度

698 °F

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

杂质

≤0.005% Chlorinated compounds
≤0.005% Sulfate (SO4)

折射率

n20/D 1.474 (lit.)

pH值（酸碱度）

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

密度

1.25 g/mL (lit.)

适用性

suitable for electrophoresis

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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一般描述

甘油是无味无色，具有粘性的甜味液体。它可以是天然来源，也可以来自石化原料。甘油具有广泛的应用，是自然界中最有价值和多功能的化学物质之一。它可用作润肤剂、溶剂、甜味剂，可用于药物配方、化妆品、食品和洗漱品中。它非常稳定，可以在常温下储存；它也无刺激性，对环境没有不良影响。

应用

甘油可用于样品制备和凝胶形成，以用于聚丙烯酰胺凝胶电泳。甘油（5-10％）可增加样品的密度，使样品层处于凝胶样品孔的底部。甘油还用于帮助制备梯度凝胶和作为蛋白质稳定剂和储存缓冲液组分。
甘油可用于样品制备和凝胶形成，以用于聚丙烯酰胺凝胶电泳。 甘油（5-10％）可增加样品的密度，使样品层处于凝胶样品孔的底部。 甘油还用于帮助制备梯度凝胶和作为蛋白质稳定剂和储存缓冲液组分。

生化/生理作用

甘油已被用作
  • 结核分枝杆菌 和 鸟分枝杆菌细胞培养过程中的补充剂；
  • 在设计酶生物燃料电池过程中的燃料；
  • 含有4-HCCA和3-氨基喹啉的液体复合基质，可用于分析中性和酸性聚糖；
  • 进行快原子轰击电离质谱的基质；
  • MALDI（基质辅助激光解吸/电离质谱）定量分析研究的液体基质。
甘油本质上是吸湿的，并且由于其具有三个亲水性醇羟基而可溶于水。它可以形成分子间和分子内氢键，使其成为一种非常灵活的分子。 甘油的生理作用是由于其在细胞介导的免疫、增加的IgG产生、以及增加的组胺释放中的影响。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

390.2 °F - Pensky-Martens closed cup

闪点(°C)

199 °C - Pensky-Martens closed cup

个人防护装备

Eyeshields, Gloves

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product No. G2025 a sterile glycerol product?

    Our quality specifications for this product include cell culture testing, but the product is not sterile as it is shipped.

  6. What is the meaning of "Performance" in your specification sheet for Product No. G2025 (Glycerol)?

    The "Performance" test demonstrates that a cell freezing medium prepared using that product maintains the viability of mammalian cells after storing the cells for 5 to 7 days at -196 °C.

  7. What is the concentration of glycerol (e.g., Product No. G2025)?

    Based on a density of 1.261 g/mL for glycerol, and a molecular weight of 92.1 g/mol, the concentration of this liquid is 13.7 M.

  8. Can I autoclave glycerol (e.g., Product No. G2025) before using it (for example) for cell culture?

    Glycerol can be autoclaved for 20 minutes, as is or in a solution, generally at approximately 50%.

  9. In what solvent is glycerol (e.g., Product No. G2025) most soluble?

    Glycerol is miscible with water and alcohol at all concentrations. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., Entry# 4493.

  10. What is the viscosity of glycerol?

    The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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T W Chew et al.
Oncogene, 33(21), 2717-2727 (2013-07-03)
RAS and Rho small GTPases are key molecular switches that control cell dynamics, cell growth and tissue development through their distinct signaling pathways. Although much has been learnt about their individual functions in both cell and animal models, the physiological
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The American journal of pathology, 185(2), 356-371 (2014-12-17)
Pulmonary veno-occlusive disease (PVOD) is an uncommon form of pulmonary hypertension (PH) characterized by progressive obstruction of small pulmonary veins and a dismal prognosis. Limited case series have reported a possible association between different chemotherapeutic agents and PVOD. We evaluated
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Aberrant viral RNAs produced in infected plant cells serve as templates for the synthesis of dsRNAs. The derived virus-related small interfering RNAs (siRNA) mediate cleavage of viral RNAs by post-transcriptional gene silencing (PTGS), thus blocking virus multiplication. Here, we identified
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