G2267
甘油醛-3-磷酸脱氢酶 来源于兔肌肉
lyophilized powder, ≥75 units/mg protein
别名:
D-甘油醛-3-磷酸:NAD+ 氧化还原酶（磷酸化）, GAPDH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-609-4
MDL number:
Specific activity:
≥75 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
form
lyophilized powder
specific activity
≥75 units/mg protein
composition
Protein, ≥80% biuret
foreign activity
3-Phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%, triosephosphate isomerase ≤0.1%
shipped in
wet ice
storage temp.
−20°C
Quality Level
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General description
甘油醛-3-磷酸脱氢酶(GAPDH)是一种多功能蛋白质。通常被称为糖酵解酶。
Application
兔肌中的甘油醛-3-磷酸脱氢酶可用于：
- 测定甘油醛-3-磷酸脱氢酶(GAPDH)活性
- 生成线性标准曲线，分析小鼠实验样品中GAPDH活性
- 用于抗聚合测定
- 用于纯化GAPDH研究
Biochem/physiol Actions
作为糖酵解的一部分，甘油醛-3-磷酸脱氢酶催化甘油醛-3-磷酸转化为1，3-二磷酸甘油酸。还显示它在启动细胞凋亡，转录激活和ER 向高尔基体囊泡的穿梭中具有作用<<<18,New>>>。
甘油醛-3-磷酸脱氢酶催化甘油醛-3-磷酸转化为D-甘油酸-1，3-二磷酸，作为糖酵解途径的一部分。
Physical form
含有柠檬酸盐缓冲液盐的冻干粉
Other Notes
在具有3-磷酸甘油酸磷酸激酶的偶联系统中，一个单位每分钟将 1.0μmole的3-磷酸甘油酯减少为D-甘油醛3-磷酸，pH 为7.6，温度为25℃。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Oligomerization of a molecular chaperone modulates its activity
Saio T, et al.
eLife, 7(6), e35731-e35731 (2018)
Poly ADP-Ribose Polymerase Inhibition Ameliorates Hind Limb Ischemia Reperfusion Injury in a Murine Model of Type 2 Diabetes
Long C A, et al.
Annals of Surgery, 258(6) (2013)
Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA repair
Kosova A A, et al.
The Journal of Biological Chemistry, 82(6), 643-654 (2017)
Paradoxical inhibition of glycolysis by pioglitazone opposes the mitochondriopathy caused by AIF deficiency
Benit P, et al.
EBioMedicine, 17, 75-87 (2017)
Julio A Massange-Sánchez et al.
Plants (Basel, Switzerland), 9(12) (2020-12-02)
Phosphoglycerate kinase (PGK, E.C. 2.7.2.3) interconverts ADP + 1,3-bisphospho-glycerate (1,3-bPGA) to ATP + 3-phosphoglycerate (3PGA). While most bacteria have a single pgk gene and mammals possess two copies, plant genomes contain three or more PGK genes. In this study, we
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