G2751
谷氨酸-草酰乙酸转氨酶 来源于猪心脏
Type I, ammonium sulfate suspension, 200-500 units/mg protein
别名:
L-天冬氨酸:2-酮戊二酸氨基转移酶, GOT, 天冬氨酸氨基转移酶
产品名称
谷氨酸-草酰乙酸转氨酶 来源于猪心脏, Type I, ammonium sulfate suspension, 200-500 units/mg protein
type
Type I
form
ammonium sulfate suspension
specific activity
200-500 units/mg protein
foreign activity
glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Analysis Note
由缩二脲确定的蛋白质。
Application
来自猪心脏的谷草转氨酶已用于：
- 在酶偶联试验中评价L-天冬酰胺酶活性
- 在微量滴定板格式酶基础检测中估算浆果样品中苹果酸的含量
- 作为均质缓冲液的补充，用于分离草酰乙酸(OAA)消耗系统环境下的心脏线粒体膜
谷氨酸-邻苯二甲酸来自猪心的转氨酶适用于制备苯丙氨酸。该酶的固定化形式可用于可溶性和固定化形式的反应效率和稳定性比较研究。
Biochem/physiol Actions
谷草转氨酶或天冬氨酸转氨酶(AAT)催化L-天冬氨酸和α-酮戊二酸与草乙酸和L-谷氨酸的相互转化。这种可逆的转氨酶反应依赖于5′-磷酸吡哆醛(PLP)。谷草转氨酶通过产生L-谷氨酸维持代谢的氮。血清谷草转氨酶水平升高在心肌梗死、肝脏疾病和某些肾脏疾病中都有指示意义。
General description
研究领域：细胞信号传导。谷草转氨酶见于所有组织，主要见于肝脏和骨骼肌。它是折叠型I型吡哆醛5′-磷酸(PLP)酶的原型。谷草转氨酶是一种同型二聚体，在每个亚基中含有大小结构域。
Other Notes
一个酶活性单位是指在pH 7.5、37 °C下，在 L-天冬氨酸存在时，每分钟将1.0 μmole的α-酮戊二酸转化为L-谷氨酸所需的酶量。在25 °C下，一个单位相当于约2,000 O.D.（Karmen）单位。
Physical form
含 0.05M 马来酸盐和 2.5mM α-酮戊二酸盐的 3.0M (NH4)2SO4 中的悬浮液，pH 6.0
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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