G2791
Anti-Grb-2 antibody, Mouse monoclonal
clone GRB-232, purified from hybridoma cell culture
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Conjugate:
unconjugated
Clone:
GRB-232, monoclonal
Application:
ARR, ELISA (i), ICC, IHC (p), IP, WB
Citations:
9
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GRB-232, monoclonal
form
buffered aqueous solution
mol wt
antigen 24 kDa
species reactivity
rat, mouse, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using a whole extract of cultured human acute T-cell leukemia Jurkat cells
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GRB2(2885)
mouse ... Grb2(14784)
rat ... Grb2(81504)
General description
Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer.
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).
Immunogen
synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2.
Application
Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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A M Pendergast et al.
Cell, 75(1), 175-185 (1993-10-08)
BCR-ABL is a chimeric oncoprotein that exhibits deregulated tyrosine kinase activity and is implicated in the pathogenesis of Philadelphia chromosome (Ph1)-positive human leukemias. Sequences within the first exon of BCR are required to activate the transforming potential of BCR-ABL. The
Arif Yurdagul et al.
Journal of cell science, 129(8), 1580-1591 (2016-02-26)
Oxidized low-density lipoprotein (oxLDL) accumulates early in atherosclerosis and promotes endothelial nuclear factor κB (NF-κB) activation, proinflammatory gene expression and monocyte adhesion. Like for other atherogenic factors, oxLDL-induced proinflammatory responses requires integrin-dependent focal adhesion kinase (FAK, also known as PTK2)
B-cell positive selection and peripheral homeostasis.
John G Monroe
Immunological reviews, 197, 5-9 (2004-02-14)
R J Daly et al.
Oncogene, 9(9), 2723-2727 (1994-09-01)
A receptor blotting technique was used to detect SH2 domain containing epidermal growth factor receptor (EGFR) substrates that exhibited differential expression either between normal breast epithelial cells and breast cancer cells or between different human breast cancer cell lines. This
A M Tari et al.
Seminars in oncology, 28(5 Suppl 16), 142-147 (2001-11-14)
The growth factor receptor-bound protein-2 (GRB2) is essential for multiple cellular functions. Inhibition of GRB2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types. GRB2 is most well known for its ability to
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