G3548
Anti-GADD45A (12-25) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-DDIT1, Anti-GADD45, Anti-Growth arrest and DNA-damage-inducible protein GADD45 α
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
human
Citations:
10
Technique(s):
western blot: 1:500-1:2,000
Uniprot accession no.:
产品名称
Anti-GADD45A (12-25) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~18 kDa
species reactivity
human
technique(s)
western blot: 1:500-1:2,000
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GADD45A(1647)
Application
Anti-GADD45A (12-25) antibody produced in rabbit has been used in immunocytochemistry.
Anti-GADD45A antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions
GADD45A (growth arrest and DNA-damage-inducible, alpha) gene encodes a protein that belongs to a group of genes that are expressed under stressful growth arrest conditions and upon treatment with DNA-damaging agents such as methylmethane sulfonate and UV radiation. The protein mediates activation of the p38/JNK pathway via MTK1/MEKK4 kinase upon environmental stress. It inhibits entry of cells into S phase by activating DNA excision repair in vitro. It plays a crucial role in cell cycle checkpoints, DNA repair, and genome integrity maintenance.
Growth arrest and DNA damage inducible α (GADD45A) miRNAs are differentially expressed in osteosarcoma. It interacts with proliferating cell nuclear antigen (PCNA), aurora A kinase and histones. GADD45A improves the cytotoxic effect of the drug temozolomide (TMZ) in glioblastoma multiforme (GBM).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Growth arrest and DNA damage inducible α It is expressed at low levels in testis, but, at moderate level in liver, brain, heart and spleen. GADD45A shows high expression in kidney and skeletal muscle.
Immunogen
synthetic peptide corresponding to amino acids 12-25 of human GADD45A
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Analysis of miRNA-gene expression-genomic profiles reveals complex mechanisms of microRNA deregulation in osteosarcoma
Maire G, et al.
Cancer Genetics, 204(3), 138-146 (2011)
Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase
Sanchez R, et al.
The Journal of Biological Chemistry, 285(29), 22196-22201 (2010)
GADD45 proteins: central players in tumorigenesis
Tamura RE, et al.
Current Molecular Medicine, 12(5), 634-651 (2012)
Jesús M Salvador et al.
Advances in experimental medicine and biology, 793, 1-19 (2013-10-10)
The first identified Gadd45 gene, Gadd45a, encodes a ubiquitously expressed protein that is often induced by DNA damage and other stress signals associated with growth arrest and apoptosis. This protein and the other two members of this small gene family
Wei Liu et al.
International journal of molecular sciences, 14(11), 21447-21462 (2013-11-02)
Growth Arrest and DNA Damage-inducible 45 (Gadd45) and MDM2 proteins, together with p21 and p53, play important roles in cell cycle checkpoints, DNA repair, and genome integrity maintenance. Gadd45 and MDM2 were activated and transcribed instantly by UV irradiation, whereas
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