G3795
抗 砂砾素抗体，小鼠单克隆
clone JP74, purified from hybridoma cell culture
别名:
Anti-A-Kinase Anchor Protein 12, Anti-AKAP12
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
JP74, monoclonal
Application:
ICC, IP, WB
Citations:
10
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JP74, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, human
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AKAP12(9590)
mouse ... Akap12(83397)
General description
Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.
Immunogen
fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).
Application
Anti-Gravin antibody has been used in western blotting immunofluorescence.
Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.
Biochem/physiol Actions
Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .
Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
Irwin H Gelman
Frontiers in bioscience : a journal and virtual library, 7, d1782-d1797 (2002-07-23)
Scaffolding proteins are thought to facilitate the efficiency and specificity of enzyme/substrate reactions by coordinating their interaction along a cytoskeletal infrastructure in a spacial and temporal manner. Rodent SSeCKS, and its human orthologue, Gravin, seem to function as tumor suppressors
Gravin regulates centrosome function through PLK1
Colicino E G, et al.
Molecular Biology of the Cell, 29(5), 532-541 (2018)
Jeffrey W Streb et al.
The Journal of biological chemistry, 279(53), 56014-56023 (2004-10-22)
A Kinase Anchoring Protein 12 (AKAP12; also known as src-suppressed C kinase substrate (SSeCKS) and Gravin) is a multivalent anchoring protein with tumor suppressor activity. Although expression of AKAP12 has been examined in a number of contexts, its expression control
J B Nauert et al.
Current biology : CB, 7(1), 52-62 (1997-01-01)
Subcellular targeting of protein kinases and phosphatases provides a mechanism for co-localizing these enzymes with their preferred substrates. A recently identified mammalian scaffold protein, AKAP79, controls the location of two broad-specificity kinases and a phosphatase. We have identified and characterized
Jacob Wang et al.
Journal of gastroenterology, 44(9), 897-911 (2009-05-27)
As the premalignant lesion of human esophageal adenocarcinoma (EAC), Barrett's esophagus (BE) is characterized by intestinal metaplasia in the normal esophagus (NE). Gene expression profiling with microarray and serial analysis of gene expression (SAGE) may help us understand the potential
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持