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Merck
CN

G5080

Sigma-Aldrich

葡聚糖凝胶 ® G-50

Fine

别名:

Sephadex G-50细粒葡聚糖树脂, 凝胶过滤树脂

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化学文摘社编号:
9048-71-9
MDL编号:
MFCD00132771
UNSPSC代码:
23151817
NACRES:
NA.56

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质量水平

200

技术

affinity chromatography: suitable

基质活性基团

phase

溶胀

1 g swells to 9-11 mL

珠子尺寸

20-80 μm

应用

life science and biopharma

相容性

Cytiva

相关类别

一般描述

Sephadex G-50（细颗粒）葡聚糖凝胶是广泛使用的凝胶过滤树脂，设计用于分子量30,000以上生物分子的脱盐和缓冲液置换。因其胶珠较小而具有高效率。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分离范围也不同。Sephadex G-50属于G型，G型Sephadex的型号包括从小分子G-10到大分子G-75的五种型号。Sephadex G-50分为4种不同粒径，包括粗(Coarse)、中(Medium)、细(Fine)和超细(Superfine)颗粒。
Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000

应用

Sephadex® G-50 适用于：

  • 低分子量和高分子量分子的分离
  • 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
Sephadex® G-50葡聚糖凝胶适于：
  • 去除脂质体悬液中的非包埋羧基荧光素(CF)
  • 通过离心式尺寸排阻色谱纯化人MN-14单克隆抗体(mAb)
  • 对5和6部分洗脱的重组蛋白进行脱盐

特点和优势

  • 一步完成脱盐、除污染和置换新缓冲液。
  • 适于通过凝胶过滤纯化DNA，除去小分子。
  • 凝胶珠较小，扩散距离更短。
  • 经典的凝胶过滤树脂。

  • Sephadex 脱盐被认为优于透析，因为它节省时间，稀释系数低，即便使用少量样品也能恢复高活性

其他说明

G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02

法律信息

Sephadex is a registered trademark of Cytiva

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000492993
0000492993
0000487397
0000487398
0000487397

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does "G-50" mean in Sephadex®  G-50, Product G5080?

    "G" stands for gel.  "50" refers to the water regain of the gel, in this instance, 5.0 g water per g dry gel.

  4. What is the best buffer to use with Sephadex®  G-50, Product G5080?

    Sephadex® G-50 is very flexible in the buffers that can be used with it.  Essentially any common aqueous buffer can be used with Sephadex® G-50.  It is advised to filter buffers through a 0.22 micron filter before use.

  5. How stable is Sephadex® G-50, Product G5080, after swelling?

    Sephadex® is stable in water, salt solutions, organic and denaturing solvents.  If necessary, the gel can be removed from the column and sterilized by autoclaving at 120 deg C, pH 7. The pH stability is limited to low strengths and short times at extremes of pH 2-10, particularly in the acid range.  After any washes using solutions at extremes of acidic or basic pH, the Sephadex®  should be washed and reequilibrated with buffer at neutral pH as promptly as possible.

  6. How can I clean the Sephadex®  G-50, Product G5080, after use?

    To clean the gel, you can wash the gel with 2 column volumes (CV) of 0.2 M NaOH, outside of the column because the gel will swell in basic pH, or in a solution of non-ionic detergent.  Then you can rinse with water and re-equilibrate with 2-3 CV of buffer. If the resin is to be stored (longer than ~24 hours), equilibrate the resin with buffer containing an antimicrobial such as 20% ethanol or 0.02% sodium azide.

  7. What kind of separation can I expect from Sephadex®  G-50, Product G5080?

    As a general rule of thumb, Sephadex® G-50 is appropriate for the separation of molecules of ~30 kDa from molecules ~1.5 kDa

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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