Product Name
GMEM培养基, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)
运输
ambient
储存温度
2-8°C
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一般描述
Glasgow最低必需培养基是通过修改Eagle BME培养基而开发的。该培养基可用于研究影响细胞能力的遗传因素。
用于粘附肾细胞系，例如幼仓鼠肾细胞 (BHK)。
应用
Glasgow最低必需培养基已用于培养：
- Hoxb7-绿色荧光蛋白（GFP）小鼠胚胎干（ES）细胞系，用于诱导输尿管芽分化
- 小鼠E14 Tg2a胚胎干（mES）细胞
- 转导中国仓鼠卵巢（CHO）细胞表达和产生Siglec-1-Fc融合蛋白
制备说明
补充 0.292 g/L L -谷氨酰胺和胰蛋白磷酸盐肉汤溶液（T8159）100 mL/L 的培养基。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
May Sallam et al.
Organogenesis, 1-10 (2021-09-28)
Work toward renal generation generally aims either to introduce suspensions of stem cells into kidneys in the hope that they will rebuild damaged tissue, or to construct complete new kidneys from stem cells with the aim of transplanting the engineered
Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Sacha Robert et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known.
Cinzia Caprio et al.
Disease models & mechanisms (2021-02-21)
The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates
Li Ding et al.
Life science alliance, 4(3) (2020-12-31)
The RNA polymerase II (RNAPII) associated factor 1 complex (Paf1C) plays critical roles in modulating the release of paused RNAPII into productive elongation. However, regulation of Paf1C-mediated promoter-proximal pausing is complex and context dependent. In fact, in cancer cell lines
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